Abstract:

Objective To investigate the effects of HDTIC-1 and HDTIC-2 isolated from Astragali Radix on the expression of p16 and p21 of human fetal lung diploid fibroblasts(2BS) so as to explore the mechanism of HDTIC delaying replicative senescence. Methods To analyse the expression levels of p16 and p21 of 2BS cells cultured with DMEM containing HDTIC by RT-PCR and western blot. Results 1)There was no expression of p16 in PD 45 2BS cells incubated with HDTIC.In PD 56 2BS cells cultured with HDTIC, there was a weak mRNA expression of p16, whereas, the protein expression of p16 was not observed.However,an obvious expression of p16 was seen in the control senescent cells. 2)For the cells cultured with HDTIC,there was a weak expression of p21 in the middle age and a strong expression of p21 in the senescent cells.Additionally,no difference of p21 level between the HDTIC-cultured cells and the control cells was seen. Conclusion There was no effect of HDTIC compounds on the expression of p21.But p16 expression of 2BS cells was strongly inhibited by HDTIC,which may contribute to their delaying replicative senescence.

Key words: astragali radix, diploid fibroblast, gene expression