Abstract: Objective To observe the effects and molecular mechanisms of a traditional Chinese medicine formula Yang Yu Tu Ji(YYTJ) on the adhesion of leukocytes to human umbilical vein endothelial cells(HUVEC),and study the mechanisms of YYTJ in promoting skin wound healing.Methods YYTJ at 132.5 μg/mL,which was confirmed to have no toxic effect on cells activity was selected as the high dose to observe the effect on HUVEC and human peripheral polymorphonuclear(PMN) leukocyte activity by MTT and Trypan-blue assay respectively.The effect of YYTJ at high(132.5 mg/L),medium(66.3 mg/L)and low(33.2 mg/L)concentration on the adhesion of PMN/THP-1 to HUVEC primed by TNF were observed by Rose Bengal staining.The expression of ICAM-1,VCAM-1 and CD44 on HUVEC were measured by fluorescent-immunocytochemistry assay.Results High concentration of YYTJ reduced PMN/THP-1-HUVEC adhesion at 12 h after treating with YYTJ and TNF,showed lower level of ICAM-1 and CD44 on HUVEC(P<0.05).Medium dose of YYTJ decreased the level of VCAM-1 and CD44 on HUVEC(P<0.05,P<0.01 respectively).Low dose of YYTJ inhibited the level of CD44 on HUVEC(P<0.05).Conclusion It's possible that YYTJ inhibit adhesion of leukocytes to HUVEC by decreasing the expression of adhesion molecules on HUVEC,therefore inhibit excessive inflammatory response.

Key words: adhesion molecules, wound healing, human umbilical vein endothelial cells(HUVEC), external herb