Effects of flavonoid-rich diet intervention on body oxidative damage related biomarkers in the health subjects with different GST genotype

doi:10.3969/j.issn.1006-7795.2013.05.014

Abstract

Abstract:

Objective To study the response of body oxidative damage related biomarkers to flavonoid-rich diet intervention in healthy subjects with different GST genotypes. Methods Totally 26 healthy adults (21~23 years old) with the GSTT1-/GSTM1 -or GSTT1+/GSTM1 + genotype were enrolled to participate in the flavonoid-rich diet intervention. The venous blood and urine samples were collected at the baseline, 1 and 2 weeks after the dietary intervention. Experimental kits were used for detecting the plasma MDA, GSH levels, and erythrocyte SOD, GST and GSH-Px enzyme activities. ELISA Kit was applied to detect the urinary 8-OH-dG level. Results No significant differences of plasma MDA, GSH levels and erythrocyte SOD, GST and GSH-Px activities were found at the baseline between GSTT1/GSTM1 genotype groups. After dietary intervention for 1 week, the plasma MDA level in GSTM1+/GSTT1+ group had a significant decrease than the baseline and that in GSTM1-/GSTT1- group, while, after intervention for 2 weeks, the MDA level in GSTT1-/GSTM1- group began to decrease to lower than that in the baseline. Two weeks of flavonoid-rich food intervention had no effects on the plasma GSH level and erythrocyte SOD enzyme activity, but increase in GSH-Px activity was found in both groups. Significant increase in GSH-Px activities were detected in GSTM1+/GSTT1+ and GSTM1-/GSTT1- groups. The response of erythrocyte GST activity was GST genotype dependent. Dietary intervention significantly increased erythrocyte GST activity in the GSTM1+/GSTT1+ group; however, no influence was detected in GSTM1-/GSTT1 -group. The content of urinary 8-OH-dG presented a decrease trend after dietary intervention for 2 weeks, however, no statistical significance was found. Conclusion Oxidative damage related biomarkers of the subjects with different GSTT1/GSTM1 genotypes had different responses to the flavonoid-rich diet.

Key words: GSTT1/GSTM1, flavonoid, dietary intervention, antioxidative capacity

CLC Number:

R151.4

YUAN Linhong, MA Weiwei, LI Nan, JI Jian, FENG Jinfang, XIAO Rong. Effects of flavonoid-rich diet intervention on body oxidative damage related biomarkers in the health subjects with different GST genotype[J]. Journal of Capital Medical University, 2013, 34(5): 704-709.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.05.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I5/704

References

[1] Bolt H M, Thier R. Relevance of the deletion polymorphisms of the glutathione-S-transferases GSTT1 and GSTM1 in pharmacology and toxicology [J]. Curr Drug Metab, 2006, 7(6): 613-628.
[2] Meijerman I, Beijnen J H, Schellens J H. Combined action and regulation of phase Ⅱ enzymes and multidrug resistance proteins in multidrug resistance in cancer [J].Cancer Treat Rev, 2008, 34(6): 505-520.
[3] Gonlugur U, Pinarbasi H, Gonlugur TE, et al. The association between polymorphisms in glutathione-S-transferase (GSTM1 and GSTT1) and lung cancer outcome [J]. Cancer Invest, 2006, 24(5): 497-501.
[4] 沈波, 许旭艳,陈艳,等.我国人群谷胱甘肽转移酶类基因多态性与慢性苯中毒易感性的Meta 分析[J]. 预防医学论坛, 2008,14(1):2-4.
[5] da Fonseca R R, Johnson W E, O'Brien S J, et al. Molecular evolution and the role of oxidative stress in the expansion and functional diversification of cytosolic glutathione transferases [J]. BMC Evol Biol, 2010, 10:281.
[6] Ansari-Lari M, Saadat M, Hadi N. Modulation of hematology changes by polymorphism of glutathione-S-transferase M1 and T1 [J]. Biochem Biophys Res Commun, 2003, 312(2):299-302.
[7] Lu M F, Xiao Z T, Zhang H Y. Where do health benefits of flavonoids come from? Insights from flavonoid targets and their evolutionary history [J]. Biochem Biophys Res Commun, 2013, 434(4):701-704.
[8] Wang C Y, Ma F L, Liu J T, et al. Protective effect of salvianic acid a on acute liver injury induced by carbon tetrachloride in rats [J]. Biol Pharm Bull, 2007, 30(1):44-47.
[9] Rusak G, Piantanida I, Masi L, et al. Spectrophotometric analysis of flavonoid-DNA interactions and DNA damaging/protecting and cytotoxic potential of flavonoids in human peripheral blood lymphocytes [J]. Chem Biol Interact, 2010, 188(1):181-189.
[10] Wilms L C, Claughton T A, de Kok T M, et al. GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro [J]. Food Chem Toxicol, 2007, 45(12):2592-2596.
[11] Wark P A, Grubben M J, Peters W H, et al. Habitual consumption of fruits and vegetables: associations with human rectal glutathione S-transferase [J]. Carcinogenesis, 2004, 25(11):2135-2142.
[12] Yuan L H, Meng L P, Ma W W, et al. The role of glutathione S-transferase M1 and T1 gene polymorphisms and fruit and vegetable consumption in antioxidant parameters in healthy subjects [J]. Br J Nutr, 2012,107(6): 928-933.
[13] 韦京豫, 徐静,郭长江,等. 北京市售30种蔬菜中黄酮类物质含量分析[J]. 中国食品卫生杂志,2009, 21(5):415-417.
[14] 杨月欣, 王光亚, 潘兴昌. 中国食物成分表 [M]. 北京, 北京大学医学出版社, 2009: 4-82.
[15] 钟诗龙, 王一西, 周书峰,等. GSTM1与GSTP1基因多态性对红细胞GST酶活性的影响 [J].中国药理学通报, 2005,21(6): 726-729.
[16] Su J F, Guo C J, Wei J Y, et al. Protection against hepatic ischemia-reperfusion injury in rats by oral pretreatment with quercetin [J]. Biomed Environ Sci, 2003, 16(1): 1-8.
[17] Leelavinothan P, Kalist S. Beneficial effect of hesperetin on cadmium induced oxidative stress in rats: an in vivo and in vitro study [J]. Eur Rev Med Pharmacol Sci, 2011, 15(9):992-1002.
[18] Zhao L, Wu J, Yang J, et al. Dietary quercetin supplementation increases serum antioxidant capacity and alters hepatic gene expression profile in rats [J]. Exp Biol Med (Maywood), 2011, 236(6):701-706.
[19] Moon Y J, Wang X, Morris M E. Dietary flavonoids: effects of xenobiotic and carcinogen metabolism [J]. Toxicol in Vitro, 2006, 20(2):187-210.
[20] Jacob R A, Aiello G M, Stephensen C B, et al. Moderate Antioxidant supplementation has no effect on biomarkers of oxidant damage in healthy men with low fruit and vegetable intakes [J]. J Nutr, 2003, 133(3): 740-743.
[21] van den Berg R, van Vliet T, Broekmans W M, et al. A vegetable/fruit concentrate with high antioxidant capacity has no effect on biomarkers of antioxidant status in male smokers [J]. J Nutr, 2001, 131(6): 1714-1722.