Roles of p75NTR inhibitor on retinal ganglion cells in a rat model of acute ocular hypertension

doi:10.3969/j.issn.1006-7795.2017.01.005

Journal of Capital Medical University ›› 2017, Vol. 38 ›› Issue (1): 24-28.doi: 10.3969/j.issn.1006-7795.2017.01.005

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Roles of p75^NTR inhibitor on retinal ganglion cells in a rat model of acute ocular hypertension

Wang Xiaolei¹, Ma Jianmin², Meng Zhaoyang¹, Yin Yi¹, Wang Yanling¹

1. Department of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Ophthalmology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2016-11-28 Online:2017-01-21 Published:2017-01-20
Supported by:
This study was supported by Clinical-Basic Cooperation Program from Capital Medical University(14JL32),Beijing Ophthalmology and Visual Sciences Key Laboratory (2015YKSJ02).

Abstract

Abstract: Objective To explore the role of p75^NTR inhibitor on suppressing retinal ganglion cells (RGCs) apoptosis in a rat model of acute ocular hypertension. Methods The acute ocular hypertension (AOH) rat model was established, and animals were divided into control (Ctrl), sham operation, AOH, AOH+TAT-Pep5 treated groups (1,3 and 5d subgroups). Immunofluorescent staining was performed to detect the expression of proNGF and p75^NTR. Western blotting was used to detect the protein expression levels of proNGF and cleaved-caspase 3. TUNEL assay was used to detect cell apoptosis. Results The expression of proNGF was increased in acute ocular hypertension model. When the p75^NTR was blocked by its inhibitor (TAT-Pep5), the number of death RGCs (TUNEL positive cell number) were less than that of AOH group. The results of Western blotting showed that the protein expression levels of cleaved-caspase 3 could be up-regulated in retina from AOH group or down-regulated in retina from AOH+TAT-Pep5 group when compared with that of Ctrl and AOH groups. Conclusion RGCs injury can increase proNGF expression. p75^NTR blockade can potentiate the survival of RGCs.

Key words: acute ocular hypertension, p75^NTR, proNGF, TAT-Pep 5 inhibitor, Müller cell

CLC Number:

R775

Wang Xiaolei, Ma Jianmin, Meng Zhaoyang, Yin Yi, Wang Yanling. Roles of p75^NTR inhibitor on retinal ganglion cells in a rat model of acute ocular hypertension[J]. Journal of Capital Medical University, 2017, 38(1): 24-28.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.01.005

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Roles of p75^NTR inhibitor on retinal ganglion cells in a rat model of acute ocular hypertension

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