Effects of major facilitator superfamily domain containing 2A on the cell proliferation, apoptosis and invasion of human hepatocellular carcinoma

doi:10.3969/j.issn.1006-7795.2021.06.016

Abstract

Abstract: Objective To explore the effects of major facilitator superfamily domain containing 2A (MFSD2A) on cell proliferation, apoptosis and invasion of hepatocellular carcinoma. Methods Hepatocellular cancer cell lines HepG2 and SMMC-7721 were transfected with lentivirus vector carried shRNA to knock down MFSD2A expression, meanwhile they were also transfected with lentivirus vector carried full length MFSD2A sequence to overexpress MFSD2A level. The qPCR and Western blotting were applied to evaluate the transfection efficiency. CCK-8 assay was used to investigate the functional effect of MFSD2A on cell proliferation. The cell apoptosis was detected with flow cytometry. The Transwell invasion assay was applied to determine the cell invasion and migration of the transfected cells. Results Upregulation of MFSD2A in hepatocellular cancer cells could significantly suppress the cell proliferation, induce the apoptosis, and decrease the cell invasion. On the contrary, downregulation of MFSD2A led to the opposite effect. Conclusion MFSD2A acted as a novel tumor suppressor gene for hepatocellular carcinoma through cell proliferation inhibition and apoptosis induction.

Key words: major facilitator superfamily domain containing 2A(MFSD2A), hepatocellular carcinoma, proliferation, apoptosis, invasion

CLC Number:

R735.7

An Songlin, Ma Ling, Zhao Yue, Li Yan. Effects of major facilitator superfamily domain containing 2A on the cell proliferation, apoptosis and invasion of human hepatocellular carcinoma[J]. Journal of Capital Medical University, 2021, 42(6): 1000-1006.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.06.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I6/1000

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