Identification of nucleic acid contamination in false positive result within detection of SARS-CoV-2 PCR

doi:10.3969/j.issn.1006-7795.2022.03.016

Journal of Capital Medical University ›› 2022, Vol. 43 ›› Issue (3): 427-432.doi: 10.3969/j.issn.1006-7795.2022.03.016

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Identification of nucleic acid contamination in false positive result within detection of SARS-CoV-2 PCR

Wang Shuang¹, Pan Yang², Xu Xinmin¹, Li Yajie¹, Zhou Chun¹, Zhang Yue¹, Wang Yajie^1*

1. Laboratory Department, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;
2. Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control, Beijing 100013, China

Received:2022-03-20 Online:2022-06-21 Published:2022-06-01
Contact: *E-mail:wangyajie@ccmu.edu.c
Supported by:
Beijing Municipal Natural Science Foundation (M21003), Capital Health Development Scientific Research Project (2021-1G-4302,2021-1G-4301).

Abstract

Abstract: Objective To explore method to identify contamination inside laboratory within 2019-nCoV nucleic acid detection. Methods Changes in amplification reaction of DNA plasmid quality control, RNA pseudovirus quality control and samples of positive patients were observed with DNase treatment recombined with removal of reverse transcription. Results DNase treatment of DNA plasmid type positive quality control with four dilutions of 1∶5, 1∶25, 1∶125 and 1∶625 longer than 15 minutes had a good scavenging effect but had little effect on RNA type. It could be used to verify DNA type contamination. The removal of reverse transcription step had a significant impact on the target gene amplification of RNA pseudovirus quality control products but had no effect on DNA components. It could be used to identify RNA contamination. Conclusion The types of contaminated components could be identified and verified with combination of DNase treatment with removal of reverse transcription in the amplification reaction.

Key words: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), false positive, laboratory contamination, identification

CLC Number:

R563.1

Wang Shuang, Pan Yang, Xu Xinmin, Li Yajie, Zhou Chun, Zhang Yue, Wang Yajie. Identification of nucleic acid contamination in false positive result within detection of SARS-CoV-2 PCR[J]. Journal of Capital Medical University, 2022, 43(3): 427-432.

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Identification of nucleic acid contamination in false positive result within detection of SARS-CoV-2 PCR

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