Abstract: Objective To investigate the characteristics of plasma high-density lipoprotein(HDL) subfraction HDL2b changes and their significance in prediction of risks for coronary heart disease(CHD) in patients with type 2 diabetes mellitus(T2DM). Methods Totally 41 T2DM patients and 41 controls with normal fasting glucose matched in age, gender, body mass index(BMI), circumference of waist, circumference of hip, and blood pressure were enrolled. The controls were from a normal population aged 35~74 year-old from Nanfaxin, a town in Shunyi District, Beijing. A microfluidic chip method was adopted to measure total HDL, HDL2b and the ratio of HDL2b/HDL, and the 10-year CHD risk was evaluated. Results T2DM patients had higher fasting plasma glucose(FPG), Homeostasis model for insulin resistance(HOMA-IS), homeostasis model assessment-insulin resistant index(HOMA-IR), triglycerides(TG), total cholesterol(TC) and 10 year CHD risk than the controls(P<0.05), while HDL, HDL2b and the ratio of HDL2b/HDL were lower in the T2DM patients(P<0.01). HDL2b was found to be negatively correlated with FBG, fasting insulin(FINS), TC, TG, HOMA-IR and 10 year CHD risk. The relationship of FBG and 10-year CHD risk with HDL2b was stronger than with HDL. Conclusion T2DM patients have significantly lower HDL2b levels, higher 10-year CHD risk. HDL2b is therefore a more sensitive predictor than HDL.

Key words: type 2 diabetes mellitus, high-density lipoprotein subfractions, microfluidic chip, coronary heart disease risk