Abstract:

Objective To study the impact of nonmyocytes conditioned media(NMCM) on the biological process of apoptosis in myocytes(MC) and the relationship with the angiotensin Ⅱ receptors.Methods 1～3 day-old SD rats were used for our experiment.The single cells were separated to myocytes(MC) and nonmyocytes(NMC) and the suspension of NMC was used as nonmyocyte conditioned media(NMCM).After 96 hours culture,the myocytes were divided into 8 groups: group M: MC+new media;group C: MC+70% new media+30% NMCM;group L: MC+new media +Losartan(10^-6mol/L);group CL: MC+70% new media +30% NMCM+Losartan;group P: MC+ new media+ PD123319(10^-6mol/L);group CP: MC+ 70% new media +30% NMCM +PD123319;group LP: MC+new media+Losartan+PD123319;group CLP: MC+ 70% new media+30%NMCM+ Losartan+ PD123319.Results 1)The area of myocytes,RNA and protien of group C,CP and CLP were greater than those of group M;2)The rates of apoptosis of group C and CP were higher than those of other groups.Conclusion NMCM made myocytes of rats hypertrophy,but not proliferation.Losartan can inhibit both NMCM-induced hypertrophy of myocytes partly and NMCM-induced apoptosis of myocytes.It is suggest that AT1 receptor can promote apoptosis of myocytes.

Key words: myocyte, nonmyocyte, apoptosis