Effects of Hypoxia on PTEN Phosphorylation and Nuclear Localization

doi:10.3969/j.issn.1006-7795.2011.02.016

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (2): 244-248.doi: 10.3969/j.issn.1006-7795.2011.02.016

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Effects of Hypoxia on PTEN Phosphorylation and Nuclear Localization

ZHOU Yun-ying¹, LIU Hua¹, GUO Rui-han², YANG Long-yan¹, BIAN Wei-hua¹, HE Jun-qi^1*

1. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Capital Medical University;2. Fiveyear Program of Basic Medical Classes, Major in Basic Medical Science, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-21 Published:2011-04-21
Contact: HE Jun-qi

Abstract

Abstract:

Objective To investigate the effects of hypoxia on endogenous phosphatase and tension homolog deleted on chromosome ten(PTEN) phosphorylation and nuclear localization.
Methods COS-7 cells were cultured in hypoxic condition(1% O₂) and harvested at 0, 1, 8, 24, 32 h. Each lysate was used to detect p-PTEN and PTEN downstream molecule p-Akt expression by Western blotting. Subcellular localization of PTEN was observed at 0, 24 h with hypoxic treatment by nucleus and plasma separation method and confocal microscopy respectively.
Results Upon hypoxic treatment for 24 h, phosphorylated PTEN level decreased significantly，accompanied by p-Akt expression level decrease; and nuclear PTEN level increase.
Conclusion In response to hypoxia treatment, PTEN was activated due to decrease of phosphorylated PTEN level, then PTEN inhibited downstream Akt activation. Under hypoxic condition, PTEN was promoted to enter nucleus.

Key words: phosphatase and tension homolog deleted on chromosome ten, hypoxia, phosphorylation, subcellar distribution

CLC Number:

Q 71

ZHOU Yun-ying, LIU Hua, GUO Rui-han, YANG Long-yan, BIAN Wei-hua, HE Jun-qi. Effects of Hypoxia on PTEN Phosphorylation and Nuclear Localization[J]. Journal of Capital Medical University, 2011, 32(2): 244-248.

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Effects of Hypoxia on PTEN Phosphorylation and Nuclear Localization

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