Long-term model, administration pattern and efficacy description of rats in acute ischemic stroke condition

doi:10.3969/j.issn.1006-7795.2014.01.023

Abstract

Abstract:

Objective To establish rat model of acute ischemic stroke of long-term symptom onset, as well as to evaluate therapeutic efficacy and side effects. Methods Individually neurological ratings were scored and self-contrast was performed, as well as continues administration were given for 6 consecutive days. Results In vivo 1 μmol/kg JXY(Gly-Arg-Pro-Ala-Lys-Arg-Gly-Asp-Val) treated rats of acute ischemic stroke immediately, or 4, 6 and 24 h after symptom onset were cured. This advantage was further enhanced by continuously administered JXY with 1 μmol/kg initial dose. In contrast, 3 mg/kg t-PA had no such therapeutic efficacy. Conclusion Improving efficacy and limiting the neurotoxicity of t-PA are of clinical importance to thrombolytic therapy in the treatment of acute ischemic stroke over 3 h symptom onset. The rat models of acute ischemic stroke of 4, 6 and 24 h symptom onset have broad utility. On the model 1 μmol/kg JXY is capable of treating acute ischemic stroke rats immediately, 4, 6 and 24 h after symptom onset.

Key words: ischemic stroke, experimental model, behavior, side effect

CLC Number:

R965.1

Jiang Xueyun, Zhang Yingjie, Zhao Ming. Long-term model, administration pattern and efficacy description of rats in acute ischemic stroke condition[J]. Journal of Capital Medical University, 2014, 35(1): 108-112.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.01.023

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I1/108

References

[1] Yang G, Zhu H, Zhao M, et al. The application of tetrahydroisoquinoline-3-carbonyl-TARGD(F)F as anti-thrombotic agent having dual mechanisms of action[J]. Mol Biosyst, 2012, 8(10): 2672-2679.

[2] Gui L, Zhao M, Wang Y, et al. Synthesis, nanofeatures, in vitro thrombuslysis activity and in vivo thrombolytic activity of poly-alpha,beta-aspartyl-L-alanine[J]. Nanomedicine (Lond),2010, 5(5):703-714.

[3] Liu J, Wang Y, Yang Y, et al. Pyrolo[1,2:4,5]-1,4-dioxopyrazino[1,2:1,6]-pyrido[3,4-b]indoles: a group of urokinase inhibitors, their synthesis and stereochemistry-dependent activity[J]. Chem Med Chem, 2011, 6(12): 2312-2322.

[4] Mei S, Liu J, Zhao M, et al. From Cerius² based stereoview to mouse and enzyme: The model systems for discovery of novel urokinase inhibitors[J]. Mol Biosyst, 2011, 7(9): 2664-2669.

[5] Li N, Kang G, Gui L, et al. Novel Cu(Ⅱ)-RGD-octapeptides: synthesis, coordination mode, in vitro anti-platelet aggregation/in vivo anti-thrombotic evaluation and correlation of sequence with nano-structure[J]. Nanomedicine, 2011, 7(4): 403-409.

[6] Guluma K Z, Lapchak P A. Comparison of the post-embolization effects of tissue-plasminogen activator and simvastatin on neurological outcome in a clinically relevant rat model of acute ischemic stroke[J]. Brain Res, 2010, 1354: 206-216. [JP]

[7] Wang C X, Todd K G, Yang Y. Patency of cerebral microvessels after focal embolic stroke in the rat[J]. J Cereb Blood Flow Metab,2001,21(4):413-421.

[8] Zhang L, Zhang Z G, Zhang R L, et al. Postischemic (6-hour) treatment with recombinant human tissue plasminogen activator and proteasome inhibitor PS-519 reduces infarction in a rat model of embolic focal cerebral ischemia[J]. Stroke, 2001, 32(12):2926-2931.

[9] Gutiérrez-Fernández M, Rodríguez-Frutos B,álvarez-Grech J, et al. Functional recovery after hematic administration of allogenic mesenchymal stem cells in acute ischemic stroke in rats[J]. Neuroscience, 2011, 175:394-405.

[10] Longa E Z, Weinstein P R, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J]. Stroke, 1989, 20(1): 84-91.

[11] Encarnacion A, Horie N, Keren-Gill H, et al. Long-term behavioral assessment of function in an experimental model for ischemic stroke[J]. J Neurosci Methods,2011, 196(2):247-257.

[12] Hetze S, Romer C, Teufelhart C, et al. Gait analysis as a method for assessing neurological outcome in a mouse model of stroke[J]. J Neurosci Methods,2012, 206(1): 7-14.

[13] Traystman R J. Animal models of focal and global cerebral ischemia[J]. ILAR J, 2003, 44(2): 85-95.

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