Abstract:

Objective To explore the sensitivity of Candida parapsilosis cells grown in developing biofilm to fluconazole and the effect of different concentration of fluconazole on Candida parapsilosis biofilm formation. Methods XTT reduction assay was used to evaluate the effect of fluconazole on developing biofilm of Candida parapsilosis and the inhibition of fluconazole on Candida parapsilosis biofilm formation. Results Twelve-hours biofilms of fluconazole-susceptible strains were resistant to fluconazole. At 12 h, 24 h, and 48 h time points, biofilm formation by fluconazole-resistant strains was significantly inhibited when fluconazole(8 μg/mL) was present. Concentration of 0.5 μg/mL of fluconazole could reduce the biofilm formation by fluconazole-susceptible strains, but the concentration of fluconazole was higher(≥1 μg/mL) for fluconazole-resistant strains. Conclusion The sensitivity to fluconazole of Candida parapsilosis cells grown in developing biofilms was different. Fluconazole inhibited biofilm formation by a variety of laboratory isolated strains.

Key words: Candida parapsilosis, biofilm, fluconazole