Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects

doi:10.3969/j.issn.1006-7795.2017.05.012

Abstract

Abstract: Objective To elucidate the human leukocyte antigenⅡ (HLA-Ⅱ) gene alleles associated with allergic rhinitis (AR) in house dust mites sensitive (HDM-sensitive) Han Chinese subjects. Methods Seventy-one patients with AR and 92 healthy subjects were recruited to this case control study. Frequencies of HLA were estimated by using the R statistical analysis package. Significance was evaluated using χ² test and Fisher exact test. For multiple allelic frequency comparisons, Bonferroni correction was applied. Genotyping of HLA-DRB1 and HAL-DQB1 was performed by the polymerase chain reaction sequence-based genotyping method. Results A total of 16 alleles of the HLA-DRB1 locus and 13 alleles of the HLA-DQB1 locus were genotyped in the entire study cohort; of which the frequencies of alleles DQB1*06:01:01 (P=0.010, OR=2.347, 95% CI:1.392-4.225) and DRB1*08:03:02 (P=0.012, OR=3.213, 95% CI:1.732-7.821) were significantly increased in HDM-sensitive AR patients compared to healthy controls and considered to be a risk factor for AR in Chinese Han subjects. The frequency of haplotype DRB1*08:03-DQB1*06:01 in HDM-sensitive AR group was also significantly higher than that in the control group (19% vs 4.8%, P=0.007, OR=4.232;95%CI:1.822-9.237). Conclusion HLA-DRB1*08:03:02 and HLA-DQB1*06:01:01 are associated with mites-sensitive AR and may confer a risk for development of AR in Han Chinese subjects sensitized to HDM.

Key words: allergic rhinitis, house dust mites(HDMs), human leukocyte antigen(HLA), DQB1, DRB1, gene polymorphism

CLC Number:

R765

Zhao Yanming, Wang Chengshuo, Zhao Yali, Zhang Yuan, Zhang Luo. Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects[J]. Journal of Capital Medical University, 2017, 38(5): 688-694.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.05.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I5/688

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