Abstract: Objective To study the effect of epidermal dendritic cells on the immune reaction and disease development in the skin.Methods The cells were taken from embryo umbilical blood and divided into monocytes.The monocytes were cultured with GM-CSF and IL-4 in vitro and investigated the changes in different stage.The MoDC were analyzed the HLA-DR,CD1a and CD80 antigen markers on the cell surface with FACS.The MoDC were stimulated with LPS,CGRP and LPS,simple CGRP,then the expression of IL-12 mRNA in the MoDC were tested.Results The monocytes from umbilical blood appeared dendritic cells in culture 7 days later and expressed HLA-DR,CD1a marker.Conclusion The neuropeptide CGRP played double modulation on the MoDC in expression of IL-12 mRNA.

Key words: dendritic cell, culture in vitro, neuroimmuno modulation