Abstract: Objective To establish a speed convenient and susceptive diagnosed method by detecting the invasion associated genes of shigella.Methods PCR and multiplex PCR was used to detect the pathogenic genes,ipaH and ial with the same time at one PCR reaction.Amplified DNA products of interest were sequenced,then the resulting was analyzed by use of the BLAST suite of programs.The sequences identified in the present databases available over the Internet were subsequently aligned.Results All 40 isolates from diarrhea stools were 14 positive for both ipaH and ial.Comparing with the routine methods the positive rate of the multiplex-PCR is high more than 4 times.Conclusion The method of PCR to detect the invasion associated genes of shigella is simple,special,sensitive,quickly and saving time.This new method can be widely used to clinic.

Key words: diarrhea, multiplex PCR, shigella