Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (4): 428-431.

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Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

Jia Hongxiao^1,3, Ma Xin¹, Jiang Yong², Ruan Yan³, Zhang Jizhi¹, Tu Pengfei², Zhang Dai³

1. Beijing Anding Hospital, Capital University of Medical Sciences;2. School of Pharmaceutical SciencesPeking University;3. Mental Health Institute, Peking University

Received:2005-06-30 Revised:1900-01-01 Online:2005-08-24 Published:2005-08-24

Abstract

Abstract:

Objective To investigate the inhibitory effect of tenuigenin on beta amyloid protein(Aβ) which caused Alzheimer's Disease(AD).Methods Western blotting was used to test the Aβ and C99 levels of cells; MTT and LDH was used to test the neurotoxicity of tenuigenin to the cells.Results Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, didn't have neurotoxicity to cells; it can decrease Aβ and C99 levels of SH-SY5Y APP695 cells, but it didn't decrease Aβ and C99 levels of SH-SY5Y SPA4CT cells.Conclusion Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, it's mechanism may come through affecting the β-secretase proteolytic activities.

Key words: Alzheimer disease(AD), amyloid &beta, -peptides(A&beta, ), C99, Western blotting

CLC Number:

R749.0+6

Jia Hongxiao;Ma Xin;Jiang Yong;Ruan Yan;Zhang Jizhi;Tu Pengfei;Zhang Dai. Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA[J]. Journal of Capital Medical University, 2005, 26(4): 428-431.

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Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

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