Abstract: Objective Thiencynonate(TC) is a novel compound which is similar to anti-cholinergic drug developed by Institute of Pharmacology and Toxicology, used for the prevention and treatment of motion sickness or other disease caused by imbalance of function under movement stimulation and the pesticides poison of organic phosphorus. Methods For further study on it, the mass spectrometry techniques of electron ionization mass spectrometry(EI-MS) and electron spray ionization mass spectrometry(ESI-MS) were used for the elucidations of the structure and fragmentation pathway of TC. Results The results shows the molecular ion peak m/z 364[M+H]⁺, m/z156, were detected by ESI-MS; m/z363[M]⁺ and m/z138,m/z181 and m/z154, m/z221 were detected by EI-MS. Further more, the fragmentation pathway of TC was also explained. Conclusion The results provided a valuable information for the investigation and development of drugs similar to TC.

Key words: mass spectrometry, thiencynonate, electron ionization mass spectrometry, electron spray ionization mass spectrometry