DL0805 inhibits the vasoconstriction induced by angiotensin Ⅱ in a Ca2+-dependent and Ca2+-independent manner in the rat aorta

doi:10.3969/j.issn.1006-7795.2013.03.015

Abstract

Abstract:

Objective To investigate the mechanisms of DL0805, a Rho kinase inhibitor, in the relaxation on the contraction induced by angiotensin Ⅱ(Ang Ⅱ) in the rat thoracic aorta. Methods Intracellular ［Ca²⁺］ (［Ca²⁺］i) was measured with Fura2/AM in vascular smooth muscle cell(VSMC). Protein level of Rho kinase 1(ROCK1), the phosphorylation levels of myosin phosphatase target subunit 1 (MYPT1) and Akt in the rat aortic rings were detected by Western blotting. Results DL0805 (1, 10 μmol/L) were shown to inhibit both Ang Ⅱ(100 nmol/L)-induced Ca²⁺ release from internal stores and Ca²⁺ influx. DL0805 (25、50 μmol/L) significantly attenuated the increased protein level of ROCK1, the increased phosphorylation levels of MYPT1 and Akt in the aorta stimulated by Ang Ⅱ(100 nmol/L). Conclusions DL0805 inhibits the vasoconstriction induced by Ang Ⅱ in a Ca²⁺-dependent and Ca²⁺-independent manner in the rat thoracic aorta.

Key words: DL0805, angiotensin Ⅱ, rat thoracic aortic rings, intracellular calcium, Rho kinase, vascular smooth muscle cell

CLC Number:

R 966

LI Li, YUAN Tianyi, GUO Jing, FANG Lianhua, YANG Haiguang, ZHANG Li, DU Guanhua. DL0805 inhibits the vasoconstriction induced by angiotensin Ⅱ in a Ca²⁺-dependent and Ca²⁺-independent manner in the rat aorta[J]. Journal of Capital Medical University, 2013, 34(3): 398-403.

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DL0805 inhibits the vasoconstriction induced by angiotensin Ⅱ in a Ca²⁺-dependent and Ca²⁺-independent manner in the rat aorta

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