Effect of sample storage method and time on the test results for serum total carbon dioxide

doi:10.3969/j.issn.1006-7795.2014.04.011

Journal of Capital Medical University ›› 2014, Vol. 35 ›› Issue (4): 438-442.doi: 10.3969/j.issn.1006-7795.2014.04.011

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Effect of sample storage method and time on the test results for serum total carbon dioxide

Du Yamei, Zhang Guojun, Chen Yan, Wang Qian, Ma Ruimin, Lyu Hong, Kang Xixiong

Clinical Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received:2014-06-18 Online:2014-08-21 Published:2014-07-22
Supported by:
This study was supported by State Science and Technology Support Program(2013BAI17B04)and High Level Technical Talent Development of Beijing Health System(2013-3-052).

Abstract

Abstract:

Objective To study the effect of sample storage method and time on the test results of serum total carbon dioxide. Methods Under the condition that total carbon dioxide(TCO₂) measurement system can guarantee the stability of TCO₂ results. TCO₂ of two quality controls and a mixed serum was measured promptly at different time points after storage under various conditions: open samples at room temperature(25±2)℃, sealed samples at room temperature, open samples at 4℃, and sealed samples at 4℃. Results High level quality control(QC), low level QC and mixed serum samples decreased by 9.65%, 13.42% and 7.74%, respectively, at 75 min when samples were stored at room temperature and kept open. The same samples decreased by 4.53, 9.43 and 3.94% at 90 min when stored at room temperature and kept seal. The samples decreased by 8.96, 8.60 and 3.94% at 75 min when stored at 4℃ and kept open, and decreased by 3.44%, 3.56% and 2.19% at 2 h when stored at 4℃ and kept sealed. Conclusion TCO₂ decreased over time and decreased at different rate according to storage condition. TCO₂ of sealed samples decreased much slower than open samples. The colder samples stored, the slower TCO₂ decreased.

Key words: serum total CO₂, automatic biochemistry analyzer, laboratory quality management

CLC Number:

R446.11

Du Yamei, Zhang Guojun, Chen Yan, Wang Qian, Ma Ruimin, Lyu Hong, Kang Xixiong. Effect of sample storage method and time on the test results for serum total carbon dioxide[J]. Journal of Capital Medical University, 2014, 35(4): 438-442.

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Effect of sample storage method and time on the test results for serum total carbon dioxide

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