S-adenosyl methionine enhance the toxicity of salsolinol in dopaminergic SH-SY5Y cell

doi:10.3969/j.issn.1006-7795.2015.06.014

Abstract

Abstract: Objective Parkinson's disease(PD) is a common neurodegenerative disease, and salsolinol which is catechol tetrahydroisoquinoline may play important role in the pathogenesis of PD. S-adenosyl methionine(SAM) may be used in the medical treatment of PD, and it is very meaningful to study the mechanism of it. Methods In our study, we chose SH-SY5Y as cell model, and used MTT to test the survival rate induced by Sal or Sal combined SAM. The apoptosis of them was detected by Hoechst 33258 stain, and the ATP level was also measured. The NMSal level which is the product of Sal N-methylation was detected by the HPLC-ECD. Results SAM can decrease the survival rate under Sal, and increased the apoptosis. The ATP level was lower in the SAM combined groups. SAM can make the cell produce more NMSal in the Sal induced groups. Conclusion These results indicated that SAM can enhance the toxicity of Sal in the dopaminergic neurons, and it may adverse to the cure of PD.

Key words: Parkinson's disease, catechol tetrahydroisoquinoline, S-adenosyl methionine, salsolinol

CLC Number:

R592

Lu Jianqing, Deng Yulin. S-adenosyl methionine enhance the toxicity of salsolinol in dopaminergic SH-SY5Y cell[J]. Journal of Capital Medical University, 2015, 36(6): 915-920.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.06.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I6/915

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