Abstract: The in vitro culture of adult human and rat's chromaffin cell was succeeded by the authors. Collagenase should be used for dissociation, because both tissues were dense. Chromaffin cells showed lower attachment ability then other cells. It may be improved by coating the culture dishes with polysine or collagen. NGF could enhance the attachment, Survival and neurite outgrowth, however, there appeared species differences. The presence of NGF in medium could enhance the survrval of rat chromaffin cells, prolong Survival time, promote neurite outgrowth. The human chromaffin cell responsed to NGF in a certain degree, but the responses were not so obvious as those of the rat.

Key words: cell culture, adrenal, chromaffin cell, nerve growth factor, monoamine oxidase.