Table of Content

15 July 1990, Volume 11 Issue 3

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论著

Analysis of Free Amino Acids in Hemolymph of Aedes albopictus

Li Fengwu;Chen Peihui;Bian Yinghua;Wang Fengyun

1990, 11(3):  157-163. 

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Hemolymph was collected from female Aedes albopictus by centrifugation. Collections were made from newly emerged mosquitoes, 4days and 11 days after mosquitoes had taken blood meal from Plasmodium gallinaceum-infected and noninfected chicks The result shows that the total amount of free amino acids (FAAs) in hemolymph of female Ae albopictus decreases 4days after blood meal and increases 11 days after the same meal, but is still less than that of newly emerged mosquitoes. The decrease of 16 FAAs and increase of 2FAAs were shown in the hemolymph of mosquitoes 4days after noninfected blood meal, the decrease of 3FAAs and increase of 15 FAAs were shown 11 days after the same meal. The decrease of 14 FAAs and increase of 4FAAs were shown in hemolymph 4days after infected blood meal, the decrease of 7 FAAs and increase of 11 FAAs were shown 11 days after the same meal.

Observation on the Morphology of Oocyst and Satellite Cyst of Plasmodium yoelii nigeriensis

Chen Peihui;Shi Yunling

1990, 11(3):  164-167. 

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The morphological and struetural characteristics of developing cocyst satellite cyst of Plasmodium yoelii nigeriensis in Anopheles dirus had been observed with the transmission electron microscopy and oil emersion microscopy for lμm section stained with Giemsa's solution. The sporoblast formation began with the appearance of vacuoles just beneath the oocyst capsule this followed by the subdivision of the cytoplasm, and sporoblast for mation. The next stage was indicated by budding of sPoroblast wilh the development of sporozoite. The satellite cysts were always developing normally, The satellite cysts could form late stage of sporoblast in the 7 day-oocyst. Most of the satellite cysts developed more slowly and the number of sporozoite buis also were fewer than that of their attaching oocysts.

Analysis of the Feasibility of Calculation Method forthe Estimation of Axonal Branching Point

Lu Cuowei;He Guorui;Li Qingjin

1990, 11(3):  168-173. 

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Some central neurons project to two or more target nuclei via their branched axons. The location of bifurcation points is usually estimated by calculation of conduction time spent in their parent axons. Howevre, in the present study, no consistant and reliable estimates of the location were shown, when four formulae were used to calculate the data obtained from intra- and extra-cellular recording. The results indicate that the calculation method is inapporpriate in its rationale and not applicable for the evaluation of branching points at least in case of unitary recording fron the ceil body.

Transcellular Transport of Fluorescent Dye from Axons to Oligodendroglia in Developing Optic Nerve

Guo Chongjie;T. Samarajski;Richard C. Wiggins

1990, 11(3):  174-179. 

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A suspension of True Blue (TB) in physiological saline was injected into the posterior chamber of the eye in rats aged 7 days, 14 days, and 28 days. Animals were killed at 1, 3, 5, 7 or 9 days post injection. Axons and oligodendroglia were labeled. Fluorescence in the optic nerve was greatest near the orbit and declined toward the optic chiasm. During rapid myelination (14 days) and late myelination (28 days) period. fluorescence labeled glias were especially abundant. The labeled glia were characteristically arranged in rows running longitudinally throughout the optic nerve. Fluorescence in the glia increased from the fifth days after the injection of the dye. The evidence supports a hypothesis that the fluorescent dye entered the oligodendroglia directly by axonal transport mechanism, possibly at the paranodal, axo-glial junction.

The in Vitro Culture of Adult Human and Rat's Chromaffin Cell of Adrenal Medullary Tissue

Zhang Guofeng;Zhao Chunli;Lu Qiang;Liou Yujun

1990, 11(3):  180-185. 

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The in vitro culture of adult human and rat's chromaffin cell was succeeded by the authors. Collagenase should be used for dissociation, because both tissues were dense. Chromaffin cells showed lower attachment ability then other cells. It may be improved by coating the culture dishes with polysine or collagen. NGF could enhance the attachment, Survival and neurite outgrowth, however, there appeared species differences. The presence of NGF in medium could enhance the survrval of rat chromaffin cells, prolong Survival time, promote neurite outgrowth. The human chromaffin cell responsed to NGF in a certain degree, but the responses were not so obvious as those of the rat.

Sarcomere Length in the Sartorius Muscle of the Toad and the Frog

Chen Xiaoliang;Han Daying

1990, 11(3):  186-190. 

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The sarcomere length in the sartorius muscle of the toad and the frog was measured with the laser diffraction technique. Measuremnts were made both in vitro and in situ. results are as follows. 1. In vitro experiments: In order to compare the sarcomere length in the two species, the sartorius muscle was fixed at its length in the functional position. The sarcomre length of the fixed-length muscle(SLf)was 2.7±0.1μm (n=11)in the toad and 2.32±0.04μm(n=11)in the frog. when the sartorius muscle was stretched from slack to break, the relationship between the sarcomere length and the muscle length is not linearas expected but sigmoid in shape. 2. In situ experiments: The in vitro finding that the SLf in the frog is smaller than that in the toad was confirmed in the in situ experiments. when the laser beam was moved in the medial to lateral direction along a line bisecting the muscle, the SLf was found to increase in value in the toad, from a value of 2.59±0.07μm(n=9)at the medial margin, to a value of 2.64±0.09μm(n=9)at the midpoint and to a value of 2.71±0.08μm(n=10)at the lateral margin. The difference in SLf was not apparent in the frog.

Study of T Lymphocyte Subsets in Acute Polyradiculoneuritis

Zhao Yueran;Wang Meiling;Li Hui;Gao Tianxiang

1990, 11(3):  191-194. 

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Ficol-Hypaque separated peripheral bloo dmononuclear cells were analysed by indirect immunofluorescence assay with ortho monoclonal antibodies to T cells (OKT₃⁺), helper cells (OKT₄⁺) and suppressor cytotoxic cells (OKT₈⁺). 20 normal subjects and 23 patients with acute polyradiculoneuritis (AP) wore studied, The percentages of OKT₃⁺ and OKT₄⁺ cells were similar in the 2 groups, but the percentages of OKT₈⁺ cells was reduced to 18.96±4.24 compared with 22.28±3.15 in the the normal subjects (p <0.05). The ratio of OKT₄⁺/OKT₈⁺ cells was correspondingly increased to 2.38±0.55, compared with 1.97±0.20 in the normal subjects. These changes fell towards normal with the passage of time. The result suggests that AP might be an autoimmunity disease with cell immunity disorders.

Changes in Contents of Major and Trace Elements in Cerebrospinal Fluid of Patients with Headache

Shi Meitang;Li Ling;Lu Guowei;Men Disheng;Ding Mingchen

1990, 11(3):  195-198. 

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The in variation contents of four types of indispensable major elements: Phosphorus (P), Sodium (Na), Calcium (Ca), and Magnesium (Mg); ten types of indispensable trace elements. Iron (Fe), Strontium(Sr), Copper(Cu), Nickel (Ni), Zinc (Zn), Vanadium (V), Chromium (Cr), Manganese (Mn), Molybdenum (Mo), and Cobalt (Co), and twelve types of other elements: Lead (Pb), Titanium(Ti), Aluminium(A1), Lanthanum(La), Barium(Ba), Silicon(Si), Cerium(Ce), Ytterbium(Yb), Seandium(Sc), Silver (Ag), Cadminm (Cd), and Boron(B) in cerebrospinal fluid(CSF) was measured on 87 patients suffering from headache and 66 patients without headache. A significant increase in Ni (p <0.05), Ce, Yb, Sc, Ag, and B (p <0.01); a significant decrease in Fe (p <0.05), Na, Ca, Mg, Sr, Zn, Al, and Ba (p <0.01) and no change in P, Cu, V, Cr, Mn, Mo, C, Pb, Si, Ti, La and Cd were found in cases suffering from headache in comparison with those in headache-free eases. The results indicate that variation in many chemical elements is associated with headache- bearing patients. Its mechanism and significance remain to be studied.

The Pathological Research of the Recurrent Pulmonary Thromboembolism

Wen Xiangyun

1990, 11(3):  199-202. 

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Recurrent pulmonary thromboembolism is a rare disease. There is no prominent symptom in its early stage, but in the later period cot pulmnale can be found. The main changes in this disease are recurrent thrombosis and thrombus organization in the pulmonary artery, therefore, it can lead to different vascular obstruction. The resistence of pulmnary circulation increases following the severity of obstruction, cor pulmonale can occur and finally leading to death of the patient. Sometimes, acute embolism of a larger pulmonary artery can also be lethal. This article has summarized the pathological characteristics of the recurrent pulmonary thromboembolism after searching three patients. It shows that the damages mainly occur in the arterial system, especially large and medial arteries, and the thrombus is apt to produce organization and reca- nalization.

Hepatic Lesiens in Experimental IgA Nephropathy

Dong Xiaoli;Zheng Guofen;Wu Zhimin

1990, 11(3):  203-205. 

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Hepatic lesions in experimental IgA nephropathy induced by CC1₄ cirrhosis of rats were demonsratted by PAP immuno-histoehemical method, IgA was found in the hepatic sinusoids, Kupffer's cells, phagocytes of portal regions and isolated hepatocytes. IgA deposits appeared earlier in the liver than in the kidney. IgG was also found in the hepatic tissues.

Observation of Treatment by Using External Diaphragm Packing Instrument for Chronic Obstructive Pulmonary Disease and Chronic Pulmonary Heart Disease Patients

Dong Ping;Zhang Hongyu;Xiang Hongdi;Wang Haoyan;Zhang Minyi

1990, 11(3):  206-208. 

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The effects of the external diaphragm packing (EDP) in the treatment of 15 patients with chronic obstructive pulmonary disease (COPD) and 20 patients with chronic pulmonary heart disease was reported. The results showed that EDP is effective in treating patients with COPD, but less effective in patients with chronic pulmonary heart disease.