Journal of Capital Medical University ›› 1991, Vol. 12 ›› Issue (4): 275-278.

• 论著 • Previous Articles     Next Articles

Production of Biotin-Labeled DNA Probes of Human Papilloma Virus11,16,18,51 Types

Shan Ailinl1, He Cuizhen1, Gao Tianxian1, Zhou Lihua2   

  1. 1. Laboratory of Electron Microscope, Capital Institute of Medicine;2. Laboratory of Electron Microscope, Shandong Medical University
  • Received:1990-02-01 Revised:1900-01-01 Online:1991-10-15 Published:1991-10-15

PDF

197

Abstract: Biotin-labeled DHA probes of human papillomavirus 11,16,18,51 types are produced by the following procedures.Fitst,culture of the strains of E.Coli DH-52 which contain cloned puc-18 plasmid and plasmid amplification.Second,extraction and purification of the plasmids after alkaline denaturation.Third,enzyme digestion of the plasmids.Fourth,biotin-labeling of HPV 11,16,18,51 DNA probes.The sensitivity of HPV 11,16,18,51 DNA probes.is detected by using dot hybridization.The results is that it is 0.05 pg for HPV 11,16,0.25 pg for HPV 18,0.1 pg for HPV 51.

Key words: human papilloma virus, DNA probes, biotin-labeling