Abstract:

We first reported in literature the syuthesize of high purity and biologically active ACEI oetapeptide with solid phase method.The resulta of the study on the relationship between depressive action on blood pressure of ACEI and brain natiuretic peptide(BNP)showed that: 1)ACEI octopeptide inhibited significantly ACE level of plasma and membrane fraction of lung tissue in rats.IC₅₀ was 10μmol/L and 5 μmol/L respectively;2)although ACEI admin-istrated(60 μg/0.6 ml/kg)by iv rapidly could supress blood pressure and aldosterone con-centration of plasma in rats,icy injection of ACEI(6μg/0.6ml.kg^-)was found no effect on blood pressure;3)tiny amount of ACEI octapeptid with no influence on plasma AT-Ⅰand aldosterone concentration increased the hypotensive effect of BNP,and 4)BNP or its fragments was found inhibitive on human plasma ACE activity.We conclude that our result may play an important role in the studies of renin-angioleusin system and physio-pathology of hyperteusive diseases.

Key words: angiotensin converting enzyme, angiotensin converting enzyme inhibitor octopeptide, brain natfiuretic peptide, blood pressure