Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells

Journal of Capital Medical University ›› 1994, Vol. 15 ›› Issue (2): 109-111.

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Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells

Gai Qiwei¹, He Cuizhen¹, Yao Qingying¹, Gao Tienxiang¹, Zhou Lihua², Liu Xiaoming²

1. Laboratory of Electron Microscopy, Capital Institute of Medicine;2. Laboratory of Electron Microscopy, Shandong Medical University

Received:1993-04-02 Revised:1900-01-01 Online:1994-04-15 Published:1994-04-15

Abstract

Abstract: Using calcium phosphate precipitation technique,we transformed in vitro NIH cell line successfully with human papilloma virus(HPV)type 16 DNA. Further analysis showed that the transformed cells possessed refractile,roundish,pile-up growth morphology,and decrease of anchorage dependence(growth in semi-fluid agar),serum dependence, and density dependence.Southern blot hybridization of transformed cell DNA indicated that HPV-16 DNA was incorporated into NIH 3T3 cells. The results indicate that HPV-16 DNA is responsible for the transformation of NIH 3T3 cells.

Key words: human papilloma virus, NIH 3T3 cell, malignant transformation

CLC Number:

R373

Gai Qiwei;He Cuizhen;Yao Qingying;Gao Tienxiang;Zhou Lihua;Liu Xiaoming. Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells[J]. Journal of Capital Medical University, 1994, 15(2): 109-111.

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Human Papilloma Virus Type 16 DNA Induces Malignant Transformation of NIH 3T3 Cells

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