Detection of Human Papilloma Virus Infection of Female Genital Tract with PCR and DNA Probes Labeled with Digoxingenin

Journal of Capital Medical University ›› 1994, Vol. 15 ›› Issue (4): 279-283.

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Detection of Human Papilloma Virus Infection of Female Genital Tract with PCR and DNA Probes Labeled with Digoxingenin

Gao Tianxiang¹, Yao Qingying¹, He Cuizhen¹, Yin Hong¹, Shan Ailin¹, Song Xuehong², Yu Xiuinei², Tang Xiaonai²

1. Department of Electron Microscopy, Capital Institute of Medicine;2. Beijing Red-Cross Chaoyang Hospital, Capital Institute of Medicine

Received:1993-07-07 Revised:1900-01-01 Online:1994-10-15 Published:1994-10-15

Abstract

Abstract:

The DNA isolated from 17 genital condylomata were analyzed by using PCR, dot and Southern blot hybridization of HPV DNA probes labeled with digoxingenin. The results show that PCR in combination with dot hybridization of HPV DNA probe labeled with digoxingenin is more sensitive and specific than dot and Sothern blot hybridization without PCR amplification.

Key words: polymerase chain reaction, hybridization of nucleic acid, human papilloma virus, digoxingenin labeling, DNA probe

CLC Number:

Gao Tianxiang;Yao Qingying;He Cuizhen;Yin Hong;Shan Ailin;Song Xuehong;Yu Xiuinei;Tang Xiaonai. Detection of Human Papilloma Virus Infection of Female Genital Tract with PCR and DNA Probes Labeled with Digoxingenin[J]. Journal of Capital Medical University, 1994, 15(4): 279-283.

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Detection of Human Papilloma Virus Infection of Female Genital Tract with PCR and DNA Probes Labeled with Digoxingenin

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