Effect of EGB on Expression of Bax Protein in Apoptosis of Neurons Following Hypoxia/Reoxygenation

Journal of Capital Medical University ›› 2004, Vol. 25 ›› Issue (2): 170-173.

• 论著·基础研究 • Previous Articles Next Articles

Effect of EGB on Expression of Bax Protein in Apoptosis of Neurons Following Hypoxia/Reoxygenation

Ji Fengqing¹, Sun Haimei¹, Guo Yanru², Guo Chongjie¹, Zhao Tiande²

1. Department of Histology and Embryology, Capital University of Medical Sciences;2. China-Japan Friendship Institute of Clinical Medical Sciences

Received:2003-10-21 Revised:1900-01-01 Online:2004-04-15 Published:2004-04-15

Abstract

Abstract: The aim was to investigate the expression of Bax protein in the apoptosis of primary cultured rat cortical neurons following hypoxia/reoxygenation(H/R) and the protective role of extract gingo biloba (EGB). The cortical neurons of E16～17 d fetal rat was primarily cultured. The apoptosis model of primary cultured cortical neurons following H/R was established by using TUNEL staining and electromicroscopy. The expression of Bax protein was investigated with immunohistochemical method. H/R can cause apoptosis of primary cultured rat cortical neurons. The apoptosis cell increased with the time, reaching peak value at H 8R 18 ; EGB could decrease the percentage of apoptosis. The expression of Bax protein increased with hypoxia time, EGB could inverse the expression of Bax. Conclusion: EGB could significantly inhibit the expression of Bax. It may be one of the mechanisms of apoptosis.

Key words: primary culture, neurons, apoptosis, Bax, EGB

CLC Number:

R322.81

Ji Fengqing;Sun Haimei;Guo Yanru;Guo Chongjie;Zhao Tiande. Effect of EGB on Expression of Bax Protein in Apoptosis of Neurons Following Hypoxia/Reoxygenation[J]. Journal of Capital Medical University, 2004, 25(2): 170-173.

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Effect of EGB on Expression of Bax Protein in Apoptosis of Neurons Following Hypoxia/Reoxygenation

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