Abstract:

Objective To evaluate the charateristics of immunophenotyping in adults with acute myeloid leukemia（AML） using multi-color flow cytometry.Methods Immunophenotyping was performed with multi-color flow cytometry using CD₄₅ /SSC gating.Results The myeloid antigen of CD₁₃ and CD₃₃ was highly expressed in all patients with AML. The positive rate was 98.3 % and 85.0% respectively. The expression of CD₁₄ and CD₆₄ in patients with AML-M₄ and AML-M₅ were also high. The positive rate was 60.0%, 100.0% in AML-M₄ and 80.0%, 100.0% in AML-M₅ respectively. The expression of CD₃₄ 、CD₁₁₇ and HLA-DR in patients with AML was 59.3%, 84.3% and 71.7% respectively. CD₁₁₇ was expressed in 76.9% of patients with AML-M₃. Expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. CD₇ and CD₁₉ expression in patients with AML was 20.0% and 3.3% respectively.Conclusion The immunophenotyping results of adults with AML were more reliable with gating of CD₄₅ /SSC. Monoclonal antibodies of CD₁₃, CD₃₃ and CD₁₄ can be used to detect the immunophenotyping in AML. The expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. The expression of CD₇ lymphoid-associated antigens is relatively common in adults with AML.

Key words: leukemia, myeloid, acute, immunophenotyping, flow cytometry