Abstract: Objective To investigate the relationship between Gprotein β3 subunit gene polymorphism and the efficacy of antihypertensive treatment with amlodipine.Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the polymorphism of Gprotein β3 subunit gene in147 individuals as normotensive control and 321 hypertensive patients.48 hypertensive patients were treated with amlodipine for 4 weeks.Results 1) There were no significant differences of the GNB3 gene C825Tpolymorphism genotypes and alleles between hypertensive patients(CC28.7%, CT 52.0%, TT 19.3%(C54.7%, T 45.3%) and normotensive controls(CC27.2%, CT 46.9%, TT 25.9%, C50.7%, T 49.3%).Mean reduction in systolic blood pressure were significantly greater in CCgenotype((4.93±2.26)kPa(37.00±16.97)mmHg) than in CT+TTgenotype((2.99±1.41)kPa(22.40±10.60)mmHg)(P<0.05).Conclusion The C825Tpolymorphism of Gprotein β3 subunit is related to the efficacy of amlodipine but not to essential hypertension.

Key words: G protein β3 subunit, gene polymorphism, response, essential hypertension, amlodipine