Promoting Effects of Extracellular α-Synuclein on the Proliferation of MES23.5 Dopaminergic Neuronal Cells

Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (5): 616-619.

• 基础研究 • Previous Articles Next Articles

Promoting Effects of Extracellular α-Synuclein on the Proliferation of MES23.5 Dopaminergic Neuronal Cells

Zhang Chen^1,2, Li Xin¹, Li Yaohua¹, Han Junyan^1,2, Chen Biao¹, Yu Shun^1,2

1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital University ofMedical Sciences;2. Institute for Hypoxia Medicine, Capital University of Medical Sciences

Received:2005-09-29 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

Abstract

Abstract: Objective To study the effects of extracellular α-synuclein (α-Syn) on the proliferation of MES23.5 Dopaminergic Neuronal Cells.Methods The recombinant human α-Syn was extracellularly administrated to the MES23.5 dopaminergic neuronal cells.Immunohistochemistry and Western blot analysis were used to detect the α-Syn entered into the cells.MTT assay was used to measure cell proliferation.Results Extracellularly administered α-Syn entered into the neuronal cells and significantly promoted the proliferation of the cells in a dose-dependent manner.A specific monoclonal antibody against α-Syn blocked the entry of α-Syn into the cells and the cell proliferation.Conclusion Extracellular α-Syn promotes the proliferation of dopaminergic neuronal cells.

Key words: α-synuclein, cell proliferation, dopaminergic neuronal cell

CLC Number:

R338

Zhang Chen;Li Xin;Li Yaohua;Han Junyan;Chen Biao;Yu Shun;. Promoting Effects of Extracellular α-Synuclein on the Proliferation of MES23.5 Dopaminergic Neuronal Cells[J]. Journal of Capital Medical University, 2006, 27(5): 616-619.

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Promoting Effects of Extracellular α-Synuclein on the Proliferation of MES23.5 Dopaminergic Neuronal Cells

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