Abstract:

Objective To investigate the effects of endotoxemia on parameters of oxidative stress and NO levels of streptozocin-induced diabetic rats.Methods Experimental diabetic animal models were constructed by streptozocin injection(50 mg/kg,i.p).The endotoxemia condition was then induced by LPS(0.5 mg/kg,i.p.).Parameters related to oxidative stress such as SOD,MDA,GSH,NO and concentrations of glucose and insulin were measured at 6,12,24 and 72 h after injection of LPS.Results The levels of serum NO and MDA elevated significantly at 24 h after LPS injection(P<0.05),whereas the levels of SOD and GSH decreased significantly at 24 h after LPS injection(P<0.05).Though no significant difference was seen in blood glucose levels in these DM groups before LPS injection,they elevated at 12,24 and 72 h after LPS injection.The insulin levels decreased in diabetic rats significantly at 24 and 72 h after LPS injection.Conclusion Endotoxemia would reduce the antioxygenic ability in diabetic rats,elevate the production of NO and impair the regulatory ability to blood glucose.

Key words: diabetes mellitus, endotoxemia, lipopolysaccharide, oxidative stress, nitric oxide