Abstract: Objective To study the preparation polygene tumor vaccine and the genetherapy for lymphoma. Methods GFP were transected into A20 cells with adenovirus, lipofectin and adenovirus with lipofectin assistant, and Hela cell lines were used as control. After 48⁷2 hours, the cells were observed under the fluorescence microscope to determine the efficiency of transfection. B7-1 and CD40L expression vectors in combination were directly injected into lymphomas of A20 mice model, the histological characteristics and the cell infiltration in lymphoma were observed. Results In the A20 cells, either the cells transected by lipofectin or adenovirus alone resulted in a very low efficiency. When adenovirus transected GFP were used with lipofectin assistant, the efficiency became higher. However, it is always far lower than that in Hela's. Intratumoral injection of B7-1 and CD40L plasmids which resulted in reduction of tumor size. Morphological observation revealed inflammatory cell infiltration in the tumors, massive necrosis and localization of tumor. Conclusion It is difficult to make tumor vaccine for lymphoma by transferring the gene into the cells in vitro. Although adenovirus with lipofectin assistant attains higher transfection efficiency, it is not sufficient, the intratumoral injection may be considered as a safe and effective way of tumor vaccine administration.

Key words: lymphoma, tumor-vaccine, gene-therapy, adenovirus, lipofectin, B7-1, CD40L