Enhanced Oligomerization of α-Synuclein in Sera of Patients with Parkinson's Disease

doi:10.3969/j.issn.1006-7795.2009.05.004

Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (5): 590-592.doi: 10.3969/j.issn.1006-7795.2009.05.004

• PD的发病机制与早期诊断 • Previous Articles Next Articles

Enhanced Oligomerization of α-Synuclein in Sera of Patients with Parkinson's Disease

LI Yan¹, YIN Juan-juan¹, LI Xin¹, WU Yan-chuan², LIU Guang-wei¹, LI Yao-hua¹, YU Shun¹

1. Department of Neurobiology, Beijing Institute of Geriatrics;Xuanwu Hospital, Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education;2. Central Laboratory, Xuanwu Hospital, Capital Medical University

Received:2009-07-16 Revised:1900-01-01 Online:2009-10-21 Published:2009-10-21

Abstract

Abstract: Objective To study the oligomerization of α-Synuclein (α-Syn) in the sera of patients with Parkinson's disease (PD) and the potential mechanism for this oligomerization. Methods Recombinant α-Syn was incubated in the sera of PD patients and of normal subjects. Immunoblot analysis was used to identify the formation of α-Syn oligomers. A special ELISA method was used to evaluate the amount of α-Syn oligomers in PD and control sera. Results The oligomeric forms of α-Syn formed in the serum were dimers, trimers, tetramers, and fourteen-polymers. Compared with control sera, the sera of PD patients significantly enhanced the oligomerization of α-Syn. Reduced glutathione significantly decreased the amount of α-Syn oligomers in the sera of PD patients. Conclusion α-Syn can form different forms of oligomers in the serum. Compared with control, sera from PD patients enhance the oligomerization of α-Syn, which may be mediated by oxidization of α-Syn.

Key words: α-Synuclein, Parkinson's disease, serum, oligomer

CLC Number:

Q 189

LI Yan;YIN Juan-juan;LI Xin;WU Yan-chuan;LIU Guang-wei;LI Yao-hua;YU Shun. Enhanced Oligomerization of α-Synuclein in Sera of Patients with Parkinson's Disease[J]. Journal of Capital Medical University, 2009, 30(5): 590-592.

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Enhanced Oligomerization of α-Synuclein in Sera of Patients with Parkinson's Disease

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