Abstract:

Objective This study aimed to explore the possible effect on angiogenesis of Liang Xue Huo Xue(LXHX) Capsules, derived from a clinical effective decoction and to understand the mechanism in treating psoriasis. Methods The chorioallantoic membrane(CAM) model was established to investigate the effect of drug serum with LXHX Capsules on the angiogenesis. Abnormal hyperplasia models of human dermal microvascular endothelial cells(HDMEC) stimulated by basic fibroblast growth factor(bFGF) and phorbol-12-myristate-13-acetate(PMA) were used to survey HDMEC proliferation by the cck-8 assay, cells migration by Transwell assay and lumen formation by lumenforming assay. Results As compared with the same serum concentration of serum that did not contain LXHX, the 5%,10% and 15% serum containing LXHX capsule components significantly inhibited angiogenesis of the CAM(P<0.05, P<0.01, respectively) and HDMEC proliferation(P<0.05, P<0.01, respectively), as well as significantly inhibited HDMEC migration(P<0.01) and lumenformation(P<0.01). Conclusion The contained LXHX capsules components inhabited CAM angiogenesis, HDMEC proliferation, migration and lumenformation which indicated that LXHX capsules affect on angiogenesis and it may be one of the mechanisms of treating psoriasis with LXHX capsule.

Key words: traditional Chinese medicine, psoriasis, angiogenesis, human dermal microvascular endothelial cells