Regulatory Effect of Nitric Oxide Signal Pathway on Ciliary Motility in Mouse Nasal Epithelial Cells

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (1): 17-20.

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Regulatory Effect of Nitric Oxide Signal Pathway on Ciliary Motility in Mouse Nasal Epithelial Cells

JIAO Jian¹, WANG Hong¹, JIN Shan-zhe¹, ZHANG Luo^1,2*

1. Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Ministry of Education, Beijing Institute of Otorhinolaryngology;2. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
Contact: ZHANG Luo

Abstract

Abstract:

Objective To investigate the effects of nitric oxide(NO) signal pathway on ciliary beat frequency(CBF) in mouse nasal epithelial cells.
Methods Cultured mouse nasal epithelial cells were treated with 10 mmol/L L-arginine, the CBF was measured by high-speed digital microscopy. In addition, expression of endothelial nitric oxide synthase(eNOS), guanylate cyclase(GC) and protein kinase G(PKG) were detected by immunohistochemistry.
Results ① CBF of epithelial cells in Hank’s balanced salt solution(HBSS) control group showed no significant changes in 10 min. ② CBF increased with treatment of 10 mmol/L L-arginine at 2 min and significant changes were observed at all the time points detected. ③ Immunoreactivity to eNOS, GC and PKG were observed in cilia of epithelial cells.
Conclusion NO-cGMP-PKG signal pathway participates in the regulating mechanism of ciliary motility in mouse nasal epithelial cells.

Key words: nitric oxide, signal pathway, arginine, cilia

CLC Number:

R 76

JIAO Jian;WANG Hong;JIN Shan-zhe;ZHANG Luo;. Regulatory Effect of Nitric Oxide Signal Pathway on Ciliary Motility in Mouse Nasal Epithelial Cells[J]. Journal of Capital Medical University, 2011, 32(1): 17-20.

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Regulatory Effect of Nitric Oxide Signal Pathway on Ciliary Motility in Mouse Nasal Epithelial Cells

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