Determination of glucosamine in human plasma by LC-MS/MS

doi:10.3969/j.issn.1006-7795.2013.05.016

Abstract

Abstract:

Objective To develop a LC-MS/MS method for the determination of glucosamine in human plasma. MethodsGlucosamine was extracted with dichloromethane from the supernatant after protein precipitation with 5% sulfosalicylic acid and separated by gradient elution on an Agilent TC-C₁₈ column using acetonitrile -0.01% ammonia water as mobile phase. Detection was carried out by multiple reaction monitoring (MRM) on a 3200QTRAP LC-MS/MS system. Results The assay was linear over the range 20.0~2 000.0 ng/mL with a lower limit of quantitation of 20.0 ng/mL. The intra-and inter-day precision were less than 15%. The relative deviation was in the range -0.30%~1.69%. The recovery of glucosamine was in the range of 93.59%~106.27%.Conclusion The method is a rapid, sensitive, selective and reliable one for the determination of glucosamine in human plasma. The assay could be applied to a pharmacokinetic study in healthy volunteers.

Key words: LC-MS/MS, glucosamine, pharmacokinetics

CLC Number:

LI Pengfei, WANG Shumin, ZHANG Xi, AN Zhuoling, LIU Lihong. Determination of glucosamine in human plasma by LC-MS/MS[J]. Journal of Capital Medical University, 2013, 34(5): 715-719.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.05.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I5/715

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