Clinical importance of glucose-regulated protein 78 and glucose-regulated protein 94 expression and its correlation with prognosis in gastric cancer

doi:10.3969/j.issn.1006-7795.2014.04.024

Abstract

Abstract:

Objective This study aimed to determine the correlation of glucose-regulated protein 78(GRP78) and glucose-regulated protein 94(GRP94) expression with clinical features and its prognostic value in gastric cancer(GC). Methods Western blotting and immunohistochemistry(IHC) were used to estimate the GRP78 and GRP94 expression in GC and distal margin of resected GC tissues. The association of GRP78 and GRP94 protein expression with clinical features and GC prognosis was determined by Kaplan-Meier curves. Results GRP78 and GRP94 immunostaining dots located predominantly in the cytoplasm and plasma membrane. Both GRP78 and GRP94 were positively correlated with differentiation, and negatively with lymph node metastasis and TNM stage(P<0.05). The mean survival time of GC patients with high and low expression of GRP78 and GRP94 were 23.51±2.32 and (37.54±3.24)months, 21.31±1.46 and (40.02±2.19)months, respectively, and the overall 5 year survival rate 21.23% and 41.42%, 20.73% and 43.86%, respectively(log-rank test, P<0.01). TNM stage and GRP78 and GRP94 were independent prognostic factors of GC. Conclusion High protein expression of GRP78 and GRP94 may correlate with poor prognosis of GC.

Key words: gastric cancer, glucose-regulated protein 78, glucose-regulated protein 94, biomarker, prognosis

CLC Number:

R735.7

Wei Hua, Jing Hong, Wu Jiang, Zhang Donghang. Clinical importance of glucose-regulated protein 78 and glucose-regulated protein 94 expression and its correlation with prognosis in gastric cancer[J]. Journal of Capital Medical University, 2014, 35(4): 501-506.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.04.024

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I4/501

References

[1] Duzgun A, Bedir A, Ozdemir T, et al. Effect of dexamethasone on unfolded protein response genes(MTJ1, Grp78, Grp94, CHOP, HMOX-1) in HEp2 cell line[J]. Indian J Biochem Biophys, 2013,50(6):505-510.
[2] Wong D P, Chu J M, Hung V K, et al. Modulation of endoplasmic reticulum chaperone GRP78 by high glucose in hippocampus of streptozotocin-induced diabetic mice and C6 astrocytic cells[J]. Neurochem Int, 2013,63(6):551-560.
[3] Slotta-Huspenina J, Wolff C, Drecoll E, et al. A specific expression profile of heat-shock proteins and glucose-regulated proteins is associated with response to neoadjuvant chemotherapy in oesophageal adenocarcinomas[J]. Br J Cancer, 2013,109(2):370-378.
[4] Ho K Y, Yeh T S, Hung K F, et al. Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth[J]. Int J Mol Sci, 2013,14(7):14439-14459.
[5] Li W, Wang W, Dong H, et al. Cisplatin-induced senescence in ovarian cancer cells is mediated by GRP78[J]. Oncol Rep, 2014,31(6):2525-2534.
[6] Slotta-Huspenina J, Berg D, Bauer K, et al. Evidence of prognostic relevant expression profiles of heat-shock proteins and glucose-regulated proteins in oesophageal adenocarcinomas[J]. PLoS One, 2012,7(7):e41420.
[7] Boelens J, Jais J P, Vanhoecke B, et al. ER stress in diffuse large B cell lymphoma: GRP94 is a possible biomarker in germinal center versus activated B-cell type[J]. Leuk Res, 2013,37(1):3-8.
[8] Fu Y, Lee A S. Glucose regulated proteins in cancer progression, drug resistance and immunotherapy[J]. Cancer Biol Ther, 2006,5(7):741-744.
[9] Lee A S. GRP78 induction in cancer: therapeutic and prognostic implications[J]. Cancer Res, 2007,67(8):3496-3499.
[10] Liu B, Staron M, Hong F, et al. Essential roles of grp94 in gut homeostasis via chaperoning canonical Wnt pathway[J]. Proc Natl Acad Sci USA, 2013,110(17):6877-6882.
[11] Zheng H, Tsuneyama K, Cheng C, et al. An immunohistochemical study of P53 and Ki-67 in gastrointestinal adenoma and adenocarcinoma using tissue microarray[J]. Anticancer Res, 2006,26(3B):2353-2360.

[1]	Wang Rongrong, Tian Junping. Correlation between serum calcium and cardiovascular outcome in patients with acute coronary syndrome [J]. Journal of Capital Medical University, 2023, 44(1): 154-160.
[2]	Jia Jing, Chen Wenming, Li Yanchen. Clinical characteristics and prognostic analysis of multiple myeloma involving central nervous system [J]. Journal of Capital Medical University, 2023, 44(1): 161-166.
[3]	Yu Tianyu, Jiang Shimin, Gao Hongmei, Zou Guming, Li Wenge. Clinical and pathological risk factors for renal prognosis in patients with biopsy-proven diabetic nephropathy classified as chronic kidney disease stage 4 [J]. Journal of Capital Medical University, 2022, 43(5): 707-712.
[4]	Gu Qiang, Yu Xiaojun, Zhang Jin. Analysis of clinical characteristics and prognosis of the very old and young old patients with mild ischemic stroke [J]. Journal of Capital Medical University, 2022, 43(3): 474-479.
[5]	Zhou Qiaoyun, Huang Xiaowu, Xia Enlan, Zhao Yuting, Huang Rui. Reproductive outcomes of women treated for intrauterine adhesions following uterine artery embolization [J]. Journal of Capital Medical University, 2022, 43(3): 490-494.
[6]	Miao Zupei, Zhang Yinghua, Xiao Keling, Si Jin, Zhang Haoyu, Li Jing. Influence of obesity on the prognosis of patients with STEMI [J]. Journal of Capital Medical University, 2022, 43(2): 192-198.
[7]	Su Jiayi, Liu Chuntao, Wang Tieshan, Li Wenkun, Yang Yi, Wu Shanshan, Li Peng, Wu Jing. Correlation between serum Helicobacter pylori antibody typing and gastric mucosal lesions [J]. Journal of Capital Medical University, 2022, 43(2): 216-220.
[8]	Sun Jing, Shi Youwu, Jia Jun, Yang Ying, Sun Zhiwei, Du Feng, Yu Jing, Xiao Yanjie, Zhang Xiaodong. Prognostic value of peripheral blood inflammation-based markers in metastatic pancreatic cancer [J]. Journal of Capital Medical University, 2022, 43(2): 299-304.
[9]	Ma Zonghui, Zhang Qian, Xing Jie, Zhu Shengtao, Li Peng, Zhang Shutian, Sun Xiujing. Incidence and influence factors of endoscopic non-curative resection of early gastric cancer [J]. Journal of Capital Medical University, 2022, 43(1): 17-21.
[10]	Liu Yang, Wu Yumei, He Yue, Fan Bei, Wang Yan, Zhao Hui, Wang Ming. Rationality verification and evaluate of the 2018 International Federation of Gynecology and Obstetrics staging system for stage ⅠB cervical cancer [J]. Journal of Capital Medical University, 2021, 42(6): 1026-1032.
[11]	Wang Li, Zhang Guojun. Study on the application value of serum neuron-specific enolase in diagnosis and therapeutic effect on patients with traumatic brain injury [J]. Journal of Capital Medical University, 2021, 42(5): 715-720.
[12]	Lai Xuan, Zhang Aihua. Effect of metabolic syndrome on survival outcome of chronic kidney disease [J]. Journal of Capital Medical University, 2021, 42(4): 609-614.
[13]	Ji Jianghuai, Zhao Xiaoxiao, Li Qianpeng, An Yi, Zhao Lei, Li Dongguo. Identification of protein-coding genes regulated by aberrant methylation in glioblastoma [J]. Journal of Capital Medical University, 2021, 42(1): 71-76.
[14]	Wang Xin, Zhang Junxuan, Gao Jian, Shi Hanping. Immunonutrition improves the prognosis of patients with cancer [J]. Journal of Capital Medical University, 2020, 41(6): 1019-1024.
[15]	Li Yan. A leader in clinical research on dementia in China who guards the “soul of memory”——Professor Jia Jianping [J]. Journal of Capital Medical University, 2020, 41(5): 822-827.