Development of customized PDMS apparatus and dynamic assays for cancer cell migratory invasion

doi:10.3969/j.issn.1006-7795.2015.02.019

Abstract

Abstract: Objective To develop assays for the dynamic analyses for the migratory invasion of tumor through biocompatible polydimethylsiloxane(PDMS) materials to produce tissue culture apparatus, thus to allow simultaneous morphological and biochemical measurements. Methods An adaptive PDMS mini-culture apparatus with a separate blade was designed and manufactured. U87MG glioblastoma cells were analyzed for time-dependent migration in correlation with the transforming growth factor-beta(TGF-β) luciferase reporter activities. The cell behavior in response to hypoxia treatment was investigated and compared with the results from classic TransWell assays. Results The dynamic monitoring and determination of cell invasiveness and migration could be conveniently achieved with flexibility using the customized PDMS apparatus. By separation and collection of the subpopulations of high-mobility cells, the involved signal transduction pathways could be detected with ease and precision. Conclusion The dynamic characterization and analyses using PDMS-based customized cell culture devices can be an attractive approach to investigate the process and underlining mechanisms of cancer cell migration. The exemplary apparatus demonstrated in this study showed promising potentials for the coordinated investigation from both morphological and biochemical measurements during the tumor cell migration process.

Key words: polydimethylsiloxane, glioblastoma, migratory invasion, hypoxia, transforming growth factor-beta(TGF-β)

CLC Number:

Xing Tianyu, Niu Jing, Ding Wei. Development of customized PDMS apparatus and dynamic assays for cancer cell migratory invasion[J]. Journal of Capital Medical University, 2015, 36(2): 270-275.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.02.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I2/270

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