Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1

doi:10.3969/j.issn.1006-7795.2015.05.013

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 729-733.doi: 10.3969/j.issn.1006-7795.2015.05.013

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Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1

Zhang Yuanyuan, Li Weiyang, Yang Lin, Li Liying

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-04-21 Online:2015-10-21 Published:2015-10-20
Contact: 李丽英 E-mail:liliying@ccmu.edu.cn
Supported by:
This study was supported by National Natural Science Foundation of China (81430013), The Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

Abstract

Abstract: Objective The aim of study is to explore the role of sphingosine 1-phosphate (S1P) signaling on murine monocyte/macrophage inflammatory cytokine expression. Methods Western blotting was employed to test whether S1P receptors (S1PRs) expression in murine monocyte/macrophages. Then we detected whether S1P-mediated expression of inflammatory cytokines in murine monocyte/macrophage J774A.1 by real-time PCR. We studied regulatory mechanisms of expression of inflammatory cytokines in J774A.1 cells by intervention of S1P/S1PRs signaling with pharmacology tools. Results S1PR₂ antagonist JTE-013, S1PR₃ antagonist CAY-10444 treatment reduced expression of inflammatory cytokines in J774A.1 cells. S1PR₁ antagonist W146 has no effects on the expression of inflammatory cytokines in J774A.1 cells. Conclusion S1P up-regulates expression of inflammatory cytokines in J774A.1 cells mediated by S1PR₂ and S1PR₃.

Key words: monocyte/macrophage, sphingosine 1-phosphate, inflammatory cytokine

CLC Number:

Q291

Zhang Yuanyuan, Li Weiyang, Yang Lin, Li Liying. Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1[J]. Journal of Capital Medical University, 2015, 36(5): 729-733.

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Regulation of inflammatory cytokine expression of sphingosine 1-phosphate in murine monocyte/macrophage J774A.1

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