Morphological study on different animal models of titanium particles inducing osteolysis

doi:10.3969/j.issn.1006-7795.2021.01.007

Abstract

Abstract: Objective To establish three different osteolysis models induced by titanium particles, and to compare their morphological differences, inflammatory level, osteolysis and osteoclast count, so as to provide a better basis for the selection of osteolysis animal models. Methods Forty five female BALB/c mice were divided into three groups randomly: Titanium particles stimulate the skull osteolysis model (group A), titanium particles stimulate the balloon model group (group B) and titanium particles stimulate the balloon bone graft model group (group C).Morphologically observe three groups of models by gross observation and histopathology. The transmission electron microscope was used to observe and compare the osteolytic area of the group A and group B, and the ultrastructure of soft tissue changes in the group C. The similarities between them and the pathophysiological changes of human specimens were discussed. Results In general, there was infiltration of titanium particles on the surface of skull in group A, and the surface of bone was not smooth; there was infiltration of titanium particles, thickening of the wall of airbag, and inflammatory edema in the group B; there were infiltration of inflammatory cells between the skull block and the surrounding airbag tissue, wrapped by titanium particles, and the inflammatory reaction was obvious in group C. HE staining results: there were some inflammatory reactions around the titanium particles on the skull surface, and the bone surface was not smooth in group A; there were many kinds of inflammatory cells infiltrated in the cyst wall, and titanium particles could gather in group B; there were inflammatory cells infiltrated around the titanium particles, and osteolysis on the bone surface was seen in group C. By transmission electron microscope: titanium particles were found in the cytoplasm of osteoclasts in group A, and some lamellar osteolysis was found under the titanium particles; titanium particles were infiltrated in the cytoplasm of cells in group B, matrix tissue was scattered around the cells, and the water content in the matrix tissue was reduced; osteoclast precursor cells were proliferative and active around the bone tissue in group C, loss of bone surface. Conclusion Three kinds of animal models of osteolysis stimulated by titanium particles have different morphological manifestations, which can provide an effective experimental platform for the basic research of osteolysis around prosthesis.

Key words: animal model, osteolysis, titanium particles, air pouch

CLC Number:

R9336

Zhang Chen, Liu Zige, Song Guorui, Li Yan, Chen Desheng, Cheng Mengqi. Morphological study on different animal models of titanium particles inducing osteolysis[J]. Journal of Capital Medical University, 2021, 42(1): 37-42.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.01.007

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I1/37

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