Effects of Cornel Iridoid Glycoside on Hippocampal Neuron Survival and Apoptosisregulating Factors in Fimbria-fornix Transected Rats

doi:10.3969/j.issn.1006-7795.2011.01.015

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (1): 73-78.doi: 10.3969/j.issn.1006-7795.2011.01.015

Previous Articles Next Articles

Effects of Cornel Iridoid Glycoside on Hippocampal Neuron Survival and Apoptosisregulating Factors in Fimbria-fornix Transected Rats

DING Yue-xia^1,2,3, ZHANG Li^1,2, YE Cui-fei^1,2, WANG Wen^1,2, LI Lin^1,2*

1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University; 2. Key Laboratory for Neurodegenerative Diseases, Ministry of Education; 3. Department of Pharmacy, Yantai Yuhuangding Hospital, Yantai, Shangdong Province

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
Contact: LI Lin

Abstract

Abstract:

Objective To investigate the effects of cornel iridoid glycoside(CIG) on hippocampal neuron survival and its mechanism after brain injury in rats.
Methods Rat model of mechanical brain injury was induced by fimbria-fornix transection(FFT). CIG was intragastrically administered for 28 days after the operation. Nissl's staining was used to evaluate neuron survival in hippocampal CA1 and dentate gyrus. Protein expression of apoptosisregulating factors(including Bcl-2, Bax and cytochrome C) in the hippocampus of rats was detected by Western blotting.
Results Nissl staining showed a significant decrease in survived neurons in hippocampal CA1 and dentate gyrus of FFT model rats; the administration of CIG(20, 60 and 180 mg·kg-1) significantly increased the number of neuronal survival, compared with the model rats. The protein expression of Bcl-2 was decreased, and the expression of Bax and cytochrome C was increased in hippocampus of FFT model rats; CIG significantly increased Bcl-2 expression and decreased the expression of Bax and cytochrome C, compared with the model rats.
Conclusion CIG markedly increased hippocampal neuronal survival in FFT model rats, and its mechanism may be related to the upregulation of apoptosis-inhibiting factors and downregulation of apoptosis-promoting factors.

Key words: cornel iridoid glycoside, fimbriafornix transection, hippocampus, Nissl&rsquo, s staining, neuron survival, apoptosisregulating factors

CLC Number:

R 338.2⁺6

DING Yue-xia, ZHANG Li, YE Cui-fei, WANG Wen, LI Lin, . Effects of Cornel Iridoid Glycoside on Hippocampal Neuron Survival and Apoptosisregulating Factors in Fimbria-fornix Transected Rats[J]. Journal of Capital Medical University, 2011, 32(1): 73-78.

0
/ Save / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2011.01.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2011/V32/I1/73

[1]	Ma Denglei, Li Yanzheng, Zhu Yanqiu, Li Lin, Zhang Lan. Effects of cornel iridoid glycoside on the behavioral and pathological changes on senescence-accelerated mouse prone 8 [J]. Journal of Capital Medical University, 2021, 42(5): 754-760.
[2]	Wang Mingyang, Sun Zhengyu, Zhang Li, Li Yali, Li Lin, Zhang Lan. Effects of cornel iridoid glycoside on mitochondria damage in rats with cerebral ischemia reperfusion injury [J]. Journal of Capital Medical University, 2020, 41(3): 385-390.
[3]	Yang Cuicui, Bao Xunjie, Zhang Li, Li Yali, Li Lin, Zhang Lan. Changes of synapse-related proteins in the brain of 7-month-old and 16-month-old APP/PS1/tau transgenic mice and the effects of cornel iridoid glycoside on the AD model [J]. Journal of Capital Medical University, 2020, 41(3): 391-396.
[4]	Ma Denglei, Zhang Xu, Zhang Li, Li Yali, Zhang Lan, Li Lin. Effects of cornel iridoid glycoside on cognitive function and tau phosphorylation in rats with traumatic brain injury [J]. Journal of Capital Medical University, 2020, 41(3): 397-402.
[5]	Yang Cuicui, Bao Xunjie, Zhang Li, Li Yali, Li Lin, Zhang Lan. Effects of cornel iridoid glycoside on Alzheimer's disease-associated neuropathology in APP/PS1/Tau transgenic mice [J]. Journal of Capital Medical University, 2019, 40(4): 588-595.
[6]	Liu Yan, Chen Yujing, Chen Wenqiang, Huang Xiaobo. Effects of Yuanzhi decoction on cognitive function and apoptosis-related protein in hippocampus of rats with chronic cerebral hypoperfusion [J]. Journal of Capital Medical University, 2019, 40(3): 323-329.
[7]	Yu Wenjiao, Yang Weiwei, Li Xin, Li Xuran, Chen Min, Yu Shun. α-Synuclein reduces surface expressions of NMDA receptors and NMDA-invoked Ca²⁺ influx and inward current by upregulation of Rab5B in cultured primary hippocampal neurons [J]. Journal of Capital Medical University, 2017, 38(6): 868-873.
[8]	ZHAO Yongmei, YAN Ying, ZHAO Zhiwei. Long-term changes of amyloid β peptide 42 and nerve growth factor expression in the hippocampus of experimental diabetic mice [J]. Journal of Capital Medical University, 2013, 34(6): 851-855.
[9]	YAN Ying, LI Sen, ZHAO Zhiwei, ZHAO Yongmei. Time course changes of amyloid-β peptide-binding alcohol dehydrogenase and choline acetyl transferase in hippocampus of diabetic mice [J]. Journal of Capital Medical University, 2013, 34(1): 53-57.
[10]	ZHAO Yongmei;LÜFengyue;XU Qunyuan. Changes of astrocytes in the hippocampus of a model of intra-ventricular injection of lipopolysaccharide in the rat [J]. Journal of Capital Medical University, 2012, 33(6): 771-775.
[11]	YAN Ying, ZHAO Yong-mei, ZHAO Zhi-wei, WANG Yu-lan, LI Sen, . The Changes in Expression of Endoplasmic Reticulum Stress Related Moleculars GRP78 and CHOP in Hippocampus of Mice with Diabetic Encephalopathy [J]. Journal of Capital Medical University, 2011, 32(1): 90-94.
[12]	Shao Guo;Gao Cuiying;Lü Guowei;. The Effects of CoCl₂ Pretreatment on HIF-1α Expression in Hippocampus of Hypoxic Preconditioned Mice [J]. Journal of Capital Medical University, 2007, 28(3): 337-340.
[13]	Gao Haiying;Zhang Bingxi. Effects of Etomidate and Midazolam on Extracellular Signal-regulated Kinase1/2 Phosphorylation in Mice Hippocampal Slices [J]. Journal of Capital Medical University, 2006, 27(5): 565-568.
[14]	Yue Yun;Feng Chunsheng. Effect of General Anesthetics on Memory Formation [J]. Journal of Capital Medical University, 2006, 27(1): 132-132.
[15]	Shao Guo;Gao Cuiying;Lü Guowei. Effects of CoCl₂ Pretreatment on Tolerance of Mice Preconditioned to Hypoxia [J]. Journal of Capital Medical University, 2005, 26(3): 288-290.

Effects of Cornel Iridoid Glycoside on Hippocampal Neuron Survival and Apoptosisregulating Factors in Fimbria-fornix Transected Rats

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles 0

Metrics

Comments