Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (1): 73-78.

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Effects of Cornel Iridoid Glycoside on Hippocampal Neuron Survival and Apoptosisregulating Factors in Fimbria-fornix Transected Rats

DING Yue-xia1,2,3, ZHANG Li1,2, YE Cui-fei1,2, WANG Wen1,2, LI Lin1,2*   

  1. 1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University; 2. Key Laboratory for Neurodegenerative Diseases, Ministry of Education; 3. Department of Pharmacy, Yantai Yuhuangding Hospital, Yantai, Shangdong Province
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
  • Contact: LI Lin

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Abstract:

Objective To investigate the effects of cornel iridoid glycoside(CIG) on hippocampal neuron survival and its mechanism after brain injury in rats.
Methods Rat model of mechanical brain injury was induced by fimbria-fornix transection(FFT). CIG was intragastrically administered for 28 days after the operation. Nissl's staining was used to evaluate neuron survival in hippocampal CA1 and dentate gyrus. Protein expression of apoptosisregulating factors(including Bcl-2, Bax and cytochrome C) in the hippocampus of rats was detected by Western blotting.
Results Nissl staining showed a significant decrease in survived neurons in hippocampal CA1 and dentate gyrus of FFT model rats; the administration of CIG(20, 60 and 180 mg·kg-1) significantly increased the number of neuronal survival, compared with the model rats. The protein expression of Bcl-2 was decreased, and the expression of Bax and cytochrome C was increased in hippocampus of FFT model rats; CIG significantly increased Bcl-2 expression and decreased the expression of Bax and cytochrome C, compared with the model rats.
Conclusion CIG markedly increased hippocampal neuronal survival in FFT model rats, and its mechanism may be related to the upregulation of apoptosis-inhibiting factors and downregulation of apoptosis-promoting factors.

Key words: cornel iridoid glycoside, fimbriafornix transection, hippocampus, Nissl&rsquo, s staining, neuron survival, apoptosisregulating factors

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