Alteration of Cytosolic Calcium in mtDNAtransferred Cells ofAlzheimer’s Disease

doi:10.3969/j.issn.1006-7795.2011.01.014

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (1): 67-72.doi: 10.3969/j.issn.1006-7795.2011.01.014

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Alteration of Cytosolic Calcium in mtDNAtransferred Cells ofAlzheimer’s Disease

ZHANG Lan^1,2, LIU Fang^1,3, WAN Sui-gui^1,2, JIA Jian-ping^1,2, LI Lin^1,2*

1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University; 2. Key Laboratory for Neurodegenerative Diseases,Ministry of Education; 3. Department of Pharmacy, No.3 Hospital Affiliated to Beijing University

Received:1900-01-01 Revised:1900-01-01 Online:2011-02-21 Published:2011-02-21
Contact: LI Lin

Abstract

Abstract:

Objective To investigate the level of cytosolic free calcium in mitochondria DNA-transferred cells of Alzheimer’s disease and the influence of mitochondrial DNA(mtDNA) deficiency on calcium regulation capacity of cells.
Methods Platelets from AD patients and aged control persons were fused with mtDNA-depleted cells to develop mtDNAtransferred cell model. Cytochrome C oxidase(COX) activity was determined by microplate assay; cytosolic calcium was labeled with Fluo-3 and fluorescence density was detected with laser scan confocal microscope(LSCM) and flow cytometer(FCM).
Results COX activity was reduced in AD mtDNA-transferred cells when compared with aged control and young control. The resting cytosolic calcium was elevated in AD mtDNA-transferred cells compared with the controls and decreased when stimulated by carbonyl cyanide m-chlorophenyl hydrazone(CCCP).
Conclusion The transfer of AD mtDNA was sufficient to produce pathological changes in cytosolic calcium regulation.

Key words: Alzheimer&rsquo, s disease, mitochondria, mtDNAtransferred cells, cytochrom C, cytosolic calcium

CLC Number:

ZHANG Lan, LIU Fang, WAN Sui-gui, JIA Jian-ping, LI Lin, . Alteration of Cytosolic Calcium in mtDNAtransferred Cells ofAlzheimer’s Disease[J]. Journal of Capital Medical University, 2011, 32(1): 67-72.

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Alteration of Cytosolic Calcium in mtDNAtransferred Cells ofAlzheimer’s Disease

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