Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (6): 719-721.

• 基础研究 • Previous Articles     Next Articles

Study on Electrophysiological Properties of Endogenous Voltage-Gated Potassium Channels in Human Embryonic Kidney(HEK293) Cells

Li Jingwei1, Hu Dayi2, Li Cuilan3, Yang Jingang2   

  1. 1. Department of Cardiology, Beijing Tiantan Hospital, Capital University of Medical Sciences;2. Department of Center of Cardiovascular Disease, Beijing Tongren Hospital, Capital Universityof Medical Sciences;3. Department of Cardiology, People's Hospital, Peking University
  • Received:2004-08-26 Revised:1900-01-01 Online:2005-12-24 Published:2005-12-24

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Abstract: Objective To study electrophysiological properties of endogenous voltage-gated outward currents of potassium channels in HEK293 cells.Methods Whole-cell voltage patch clamp experiment was performed on this expression system.Results In HEK293 cells, depolarizing voltage steps from-80 mVtriggered an outward current.This current had an amplitude (422.78±68.87) pAat +100 mVand a current density of (21.91±3.20) pA/pF.TEA, 4-APas potassium channel blockers and a rise in extracellular K+ concentration from 4 to 40 mmol/Lcould block the outward currents.Conclusion Functional endogenous K+ channels exist in HEK293 cells under normal culture conditions.Their outward current may consist of IK, IK1, IKur and Ito.

Key words: HEK293 cells, patch-clamp, whole-cells recording, delayed rectifier, voltage-gated K~+ currents

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