Expression of α1A, β1 and β2 adrenergic receptors in rats lung with heart failure induced by myocardial infarction

doi:10.3969/j.issn.1006-7795.2013.01.027

Abstract

Abstract:

Objective To investigate the protein levels of α_1A,β₁ and β₂ adrenergic receptor ( AR ) in lungs of rats with chronic heart failure (CHF) induced by myocardial infarction. Methods Models of CHF established by anterior descending coronary artery ligature. Thirty five Wistar rats were randomly divided into three groups: control group (group A), sham operation group (group B) and CHF model group (group C). The protein expression levels of α_1A-AR, β₁-AR and β₂-AR in lung tissue were measured by Western blotting.Results Compared with sham operation group,α_1A-AR, β₁-AR protein expression in group C markedly decreased (P<0.01 and P<0.05), expression of β₂-AR remarkably increased (P<0.05), while there was no significant difference between control group and sham operation group (P>0.05). Conclusion CHF rats always had pulmonary congestion, the α_1A-AR down-regulation helped pulmonary vascular relax and inhibited smooth muscle cells proliferation, which was a compensation of the pathological situation of CHF lung. Increasing expression of β₂-AR in CHF lungs could relax the vascular and bronchial smooth muscle; promote the clearance of pulmonary edema fluid and lung conditions through opening the sodium ion channels and other mechanisms. It could also improve the permeability of the capillaries in lungs. The expression of β₁-AR in CHF rats was down-regulated for negative feedback regulation of activation of the sympathetic adrenergic system or compensation of up-regulation of β₂-AR,which could maintain a relatively stable rates of alveolar fluid clearance.

Key words: adrenergic receptor, lung, heart failure

CLC Number:

R541.6

JIANG Zhili, LI Yanfang, CAO Fangfang, LIU Fei, BAI Xueyuan, LÜ Yang. Expression of α_1A, β₁ and β₂ adrenergic receptors in rats lung with heart failure induced by myocardial infarction[J]. Journal of Capital Medical University, 2013, 34(1): 140-143.

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Expression of α_1A, β₁ and β₂ adrenergic receptors in rats lung with heart failure induced by myocardial infarction

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