Therapeutic effect of citalopram on depressive model rats and the relative mechanism

doi:10.3969/j.issn.1006-7795.2013.02.015

Abstract

Abstract:

Objective To explore the protective effect of citalopram on depressive model rats and the relative mechanism through continuous intraperitoneal injection of citalopram. Methods Establishing chronic unpredictable stress rat model. It consisted of a variety of stressors applied randomly every day for 4 weeks, The type of stressor included restraint, forced swim, food or water deprivation, tail pinch and so on. Successful model rats were subjected to continuous intraperitoneal injection with different doses of citalopram for 3 weeks, followed by behavioral tests. Sacrificing the rats immediately after the last behavioral test, the content of brain-derived neurotrophic factor(BDNF) in the hippocampus and serum was detected by ELISA and Western Blotting. Results After chronic unpredictable stress for 4 weeks, the model rats displayed lower weight gain, decreased sucrose preference, and reduced locomotor activity. Intraperitoneal injection with 10 mg·kg^-1·d^-1 citalopram for 3 weeks could significantly increase the sucrose preference and the movement distance of model rats. Intraperitoneal injection with 10 mg·kg^-1·d^-1 citalopram for 3 weeks could significantly increased the content of BDNF in the serum and the hippocampus of model rats. Conclusion Rats subjected to chronic unpredictable stress for 4 weeks displayed apparent depressive symptoms. Intraperitoneal injection with 10 mg·kg^-1·d^-1 citalopram for 3 weeks could improve the depressive behavior of model rats, the underlying mechanism may relate to increased BDNF content in the hippocampus.

Key words: citalopram, depression, brain-derived neurotrophic factor, chronic unpredictable stress

CLC Number:

YAO Yao, YANG Jian, PEI Yu, WANG Yong, MA Xin, WANG Xiaomin, Wang Xuan. Therapeutic effect of citalopram on depressive model rats and the relative mechanism[J]. Journal of Capital Medical University, 2013, 34(2): 239-244.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.02.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I2/239

References

[1] Lopez A D, Murray C C. The global burden of disease, 1990-2020[J]. Nat Med, 1998,4(11):1241-1243.

[2] Overo K F. Preliminary studies of the kinetics of citalopram in man[J]. Eur J Clin Pharmacol, 1978,9(14):69-73.

[3] Dwivedi Y. Brain-derived neurotrophic factor: role in depression and suicide[J]. Neuropsychiatr Dis Treat. 2009, 5:433-449.

[4] . A comparison of prednisolone with aspirin or other analgesics in the treatment of rheumatoid arthritis. A second report by the joint committee of the Medical Research Council and Nuffield Foundation on clinical trials of cortisone, ACTH, and other therapeutic measures in chronic rheumatic diseases[J]. Ann Rheum Dis, 1960,19:331-337.

[5] Berton O, Nestler E J. New approaches to antidepressant drug discovery: beyond monoamines[J]. Nat Rev Neurosci, 2006,7(2):137-151.

[6] Castren E, Rantamaki T. The role of BDNF and its receptors in depression and antidepressant drug action: reactivation of developmental plasticity[J]. Dev Neurobiol, 2010,70(5):289-297.

[7] Katz R J. Animal models and human depressive disorders[J]. Neurosci Biobehav Rev, 1981,5(2):231-246.

[8] Willner P, Towell A, Sampson D, et al. Reduction of sucrose preference by chronic unpredictable mild stress, and its restoration by a tricyclic antidepressant[J]. Psychopharmacology(Berl), 1987,93(3):358-364.

[9] Luo K R, Hong C J, Liou Y J, et al. Differential regulation of neurotrophin S100B and BDNF in two rat models of depression[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2010,34(8):1433-1439.

[10] Wurtman R J. Genes, stress, and depression[J]. Metabolism, 2005,54(5 Suppl 1):16-19.

[11] 常凤友.难治性抑郁症的增效治疗[J].中国煤炭工业医学杂志,2011,14(11):1730.

[12] 付华斌,陈峰,罗克勇,等.难治性抑郁症的研究进展[J].解放军医药杂志,2011,23(3):67-79.

[13] Willner P. Animal models as simulations of depression[J]. Trends Pharmacol Sci, 1991,12(4):131-136.

[14] Davies A M. The role of neurotrophins in the developing nervous system[J]. J Neurobiol, 1994,25(11):1334-1348.

[15] Angelucci F, Brene S, Mathe A A. BDNF in schizophrenia, depression and corresponding animal models[J]. Mol Psychiatry, 2005,10(4):345-352.

[16] 尹烈虎,李学成,陆地,等.BDNF用于抑郁症辅助诊断和疗效判断的初步研究[J].西南国防医药,2012,22(2):155-158.

[17] Matrisciano F, Bonaccorso S, Ricciardi A, et al. Changes in BDNF serum levels in patients with major depression disorder(MDD) after 6 months treatment with sertraline, escitalopram, or venlafaxine[J]. J Psychiatr Res, 2009,43(3):247-254.

[18] Piccinni A, Marazziti D, Catena M, et al. Plasma and serum brain-derived neurotrophic factor(BDNF) in depressed patients during 1 year of antidepressant treatments[J]. J Affect Disord, 2008,105(1-3):279-283.