Expression of Toll-like receptor 11~13 in mast cells

doi:10.3969/j.issn.1006-7795.2013.02.017

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (2): 251-257.doi: 10.3969/j.issn.1006-7795.2013.02.017

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Expression of Toll-like receptor 11~13 in mast cells

LI Yan^1,2, LI Bo³, YAO Xiujuan^1,2, LV Zhe², AN Yunqing², WANG Wei², HUANG Kewu¹, SUN Ying^2,4

1. Division of Pulmonary and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing Institute of Respiratory Medicine, Beijing 100020, China;
2. Department of Immunology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China;
3. Division of Risk Assessment, Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050, China;
4. Division of Asthma, Allergy & Lung Biology, King’s College London, Medical Research Council & Asthma United Kingdom Centre in Allergic Mechanisms of Asthma, London SE19RT, United Kingdom

Received:2012-12-26 Online:2013-04-21 Published:2013-04-17
Supported by:
This study was supported by National Natural Science Foundation of China(81102250,81072446), the Science and Technology Project of the Beijing Municipal Education Commission(KM201010025002, KM201110025005), Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PHR201008382)

Abstract

Abstract:

Objective To identify whether murine mast cells express Toll-like receptors 11-13(TLR11-13). Methods Reverse transcription polymerase chain reaction(RT-PCR), flow cytometry and immunofluorescent staining were used to detect the expression of TLR11-13 in murine bone marrow-derived mast cell(BMMC) and mast cell line MC/9 at the levels of mRNA and protein. Results Both BMMC and MC/9 expressed TLR11-13 at both mRNA and protein levels. Conclusion The expression of TLR11-13 by mast cells has provided clues to future investigating biological functions of these TLRs in mast cells.

Key words: mast cells, Toll-like receptor11, Toll-like receptor12, Toll-like receptor13

CLC Number:

LI Yan, LI Bo, YAO Xiujuan, LV Zhe, AN Yunqing, WANG Wei, HUANG Kewu, SUN Ying. Expression of Toll-like receptor 11~13 in mast cells[J]. Journal of Capital Medical University, 2013, 34(2): 251-257.

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Expression of Toll-like receptor 11~13 in mast cells

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