Construction of vascular depression animal model based on chronic ischemic and stress

doi:10.3969/j.issn.1006-7795.2016.02.017

Abstract

Abstract: Objective To build the ideal animal model for depression. Methods Forty SD rats were divided randomly into 4 groups: 1sham-operated group; 2operation group: bilateral common carotid artery ligation (LBCCA); 3depression group: chronic unpredictable mild stress (CUMS) combining with solitary, with no surgery; 4model group: LBCCA +CUMS and solitary. We observe animals continuously for 21 days to detect the variation of weight, behavioristics, cerebral blood flow and expression level of matrix metalloproteinases (MMPs), brain-derived neurotrophic factor (BDNF)/tropomyosin related kinase B (TrkB) and MMPs mRNA, BDNF/TrkB mRNA. Results Compared with the sham-operated group, model group rats and depression group rats were significantly decreased (P<0.05 or P<0.01) in weight, sucrose consumption, movement distance and the number of rearing in open-field test; the blood flow of rats of operation group and model group were significantly decreased (P<0.01); the expression level of MMP-2 and MMPs mRNA in model group and operation group were increased markedly (P<0.01), the expression level of BDNF/TrkB and BDNF/TrkB mRNA in model group and depression group were decreased significantly (P<0.01). Conclusion The method of LBCCA superposition CUMS combined with solitary simulate well the core symptoms of depression (lack of sensation, decline of movement and exploration) and the pathomechanism of unbalance of nerve vascular unit. It's an ideal model for vascular depression, and it can be used in the study of pharmacological experiment and the mechanism of curative effect.

Key words: vascular depression(VD), animal model, neurovascular unit, matrix metalloproteinases (MMPs), brain-derived neurotrophic factor (BDNF), tropomyosin related kinase B (TrkB)

CLC Number:

R743.31

Tan Sai, Lei Xiaoming, Wang Yanyun, Pan Juhua, Zhang Ying, Huang Shijing, Zhang Yongchao, Zheng Jun, Chen Zhao, Chen Yuxia, Li Duojiao. Construction of vascular depression animal model based on chronic ischemic and stress[J]. Journal of Capital Medical University, 2016, 37(2): 196-202.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.02.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I2/196

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