Studies of serum biomarkers to differentiate and diagnose acute pneumonia pathogens infection

doi:10.3969/j.issn.1006-7795.2016.05.016

Abstract

Abstract: Objective To provide the experimental basis of a rapid immune colloidal gold test which used to differentiate and diagnose acute pneumonia patients infected with mycoplasma pneumoniae(MP)/virus/bacteria. Methods The level of platelet-derived growth factor (PDGF) and bactericidal/permeability-increasing protein (BPI) in the serum of acute pneumonia patients with definite infection of mycoplasma pneumoniae/virus/bacteria and healthy controls were measured by enzyme-linked immunosorbent assay (ELISA) method. Results There were 78 serum samples from healthy controls, 46 from acute MP patients, 42 from acute bacterial and viral pneumonia patients (7 from bacterial pneumonia and 35 from viral pneumonia patients). (2) PDGF values of healthy controls were all less than 6 000 pg/mL; BPI values were all less than 80 ng/mL. (3) If definition of the baseline value of PDGF which could diagnose acute MP infection is as 6 000 pg/mL, the positive detection rate of MP pneumonia patients was 93.47% (43/46), and the MP false positive rate of patients with viral or bacterial pneumonia was 7.14% (3/42). (4) If definition of the baseline value of BPI which could diagnose acute bacterial infection is 80ng/mL, the positive detection rate of patients with bacterial pneumonia was 85.71% (6/7), and the bacteria false positive rate of viral pneumonia patients was 3.22% (1/31). Conclusion Using PDGF's baseline value as 6 000 pg/mL and BPI's 80 ng/mL to differential diagnosis has some clinical significance to differentiate acute MP/virus/bacterium infection.

Key words: pneumonia, differentiation diagnosis, platelet-derived growth factor, bactericidal/permeability-increasing protein

CLC Number:

R563.1

Liu Meng, Dou Yunpeng, Gu Li, Lyu Zhe, Wang Yimin, Liu Yingmei, An Yunqing, Cao Bin. Studies of serum biomarkers to differentiate and diagnose acute pneumonia pathogens infection[J]. Journal of Capital Medical University, 2016, 37(5): 636-640.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.05.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I5/636

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