Effects of Pseudomonas aeruginosa lipopolysaccharide on biofilm of Candida albicans

doi:10.3969/j.issn.1006-7795.2017.04.019

Abstract

Abstract: Objective To explore the inhibitory effects of Pseudomonas aeruginosa lipopolysaccharide on Candidas albicans biofilms. Methods ［(2,3-bis-(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide,XTT］ reduction assay was used to elucidate the effect of Pseudomonas aeruginosa lipopolysaccharide on biofilm formation of Candida albicans,and inverted microscope was used to observe the morphological changes of Candida albicans biofilms; expression analysis of Candida albicans hypha specific genes was done by quantitative real time polymerase chain reaction(PCR). Results The data showed that Pseudomonas aeruginosa lipopolysaccharide inhibits Candidas biofilm formation,and the expressions of HWP1, ALS1,ALS3,ECE1,SAP4 were downregulated 8.3 to 12.8 times,1.1 to 3.0 times,2.0 to 4.6 times,1.3 to 3.8 times and 6.2 to 7.6 times. Conclusion Pseudomonas aeruginosa lipopolysaccharide modulated Candida albicans biofilm formation by inhibiting the hyphal development.

Key words: Pseudomonas aeruginosa, lipopolysaccharide, Candidas albicans, biofilm, genes

CLC Number:

R379.4

Deng Juan, Sun Wei, Ma Liyan, Su Jianrong. Effects of Pseudomonas aeruginosa lipopolysaccharide on biofilm of Candida albicans[J]. Journal of Capital Medical University, 2017, 38(4): 586-591.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.04.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I4/586

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