Pathological mechanism study on the colitis mice model

doi:10.3969/j.issn.1006-7795.2018.05.015

Abstract

Abstract: Objective To study the pathological and immune mechanism in the ulcerative colitis(UC) mice induced by dextran sodium sulfate (DSS) that could be provide clue for the treatment. Methods We feed the mice with DSS solution for one, three, five days and the solution without DSS was as control group. Immunohistochemistry was used to detect pathological feature in colon tissues of mice; real-time PCR was performed to investigate immune factors expression. Results Via DSS solution to feed the mice with a variable of times, mouse weight decreased, length of colon shortened and spleen of mice enlarged, gradually. The result from HE staining on the colon tissues of mice with 5 days DSS feeding showed that enterelcosis existed; MPO staining demonstrated that neutrophile granulocyte widely invaded. In addition, inflammatory factor and inflammatory associated factors including tumor necrosis factor-α (TNF-α), interleukin-1α (IL-1α), IL-1β, IL-6,[chemokine(C-X-C motif) ligand 1,CXCL-1], CXCL-2 and cyclooxygenase-2 (COX-2) were significantly increased. Conclusion Using DSS solution to feed the mice with 5 days formed UC model infiltrating with a lot of inflammatory factors and chemokines, indicating occurrence and progression of UC are associated with infiltrating inflammatory factors and inhibiting these factors may control disease.

Key words: ulcerative colitis, dextran sodium sulfate, inflammatory factor, chemokine

CLC Number:

R365

Yang Min, An Guo, Zhao Wei, Ma Yuanyuan. Pathological mechanism study on the colitis mice model[J]. Journal of Capital Medical University, 2018, 39(5): 704-709.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.05.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I5/704

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