Evaluation of disinfection efficacy of levulinic acid plus sodium dodecyl sulfate for Mycobacterium tuberculosis and safety assessment in toxicology

doi:10.3969/j.issn.1006-7795.2018.06.018

Abstract

Abstract: Objective To investigate the germicidal efficacy of levulinic acid plus sodium dodecyl sulfate (LVA-SDS) for H37Rv strain and multidrug-resistant Mycobacterium tuberculosis clinical isolates and to assess the toxicologic safety of LVA-SDS in practical application. Methods According to the 2002 edition of the Technical Specification for Disinfection, suspension quantitative germicidal test and animal experiment methods were respectively used to evaluate the germicidal efficacy of LVA-SDS for H37Rv and multidrug-resistant Mycobacterium tuberculosis isolates and the toxicologic safety of LVA-SDS. Results The average killing logarithm values of 20%LVA+2%SDS for H37Rv or multidrug-resistant Mycobacterium tuberculosis isolates were respectively>5.00. The maximum of the inactivation of the disinfectant on the acute toxicity test was LD₅₀>5 000 mg·kg^-1. Cumulative coefficient K>5. No erythema and edema were found in skin irritation, and the skin irritation index was<0.5. Conclusion LVA-SDS has high efficacy for killing H37Rv and multidrug-resistant Mycobacterium tuberculosis isolates with actually non-toxic grade, and no cumulative toxicity and no skin stimulation. Therefore, LVA-SDS is a safe disinfectant for Mycobacterium tuberculosis and has strong practical application value.

Key words: Mycobacterium tuberculosis, levulinic acid(LVA), average killing logarithm values, safety

CLC Number:

R52
R979.7

Lyu Xiali, Li Zihui, Pan Liping, Jia Hongyan, Zhang Zongde, Liu Yang. Evaluation of disinfection efficacy of levulinic acid plus sodium dodecyl sulfate for Mycobacterium tuberculosis and safety assessment in toxicology[J]. Journal of Capital Medical University, 2018, 39(6): 894-899.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.06.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I6/894

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